三种不同颜色果实的红豆杉树皮中紫杉醇含量比较研究  

Comparison of Paclitaxel Content from Barks of Chinese Yew with Three Different Color of Fruits

作　　者：曹殿洁[1] 张国升[1] 卫强[1] 刘志 吴甜甜 徐帅 郝陈陈 Cao Dianjie;Zhang Guosheng;Wei Qiang;Liu Zhi;Wu Tiantian;Xu Shuai;Hao Chenchen(College of Pharmacy,Anhui Xinhua University,Hefei,23000)

机构地区：[1]安徽新华学院药学院

出　　处：《基因组学与应用生物学》2018年第11期5102-5107,共6页Genomics and Applied Biology

基　　金：2015年安徽省教育厅自然科学重点项目(No.KJ2015A313);2016年高校优秀青年人才支持计划重点项目(No.gxyqZD2016394)共同资助

摘　　要：本研究比较了三种安徽宣城采集的不同颜色果实的红豆杉树皮中紫杉醇的含量。利用三氯甲烷料液比1：5，回流提取2h，活性炭脱色后制备供试品溶液。采用HPLC法，Agilent HC—C18色谱柱（250mm×4.6mm，5μm），以甲醇-水-乙腈（24：30：36）为流动相，在227nm波长下检测紫杉醇含量。检测发现红豆杉树皮中紫杉醇含量为0.000564mg／g，黄色果实红豆杉树皮中紫杉醇含量为0．000785mg／g，金黄色果实红豆杉树皮中紫杉醇含量为0．000703mg／g。安徽宣城发现的黄色果实红豆杉和金黄色果实红豆杉树皮中的紫杉醇含量比红豆杉高，初步明确了黄色果实红豆杉的药用价值，为进一步发掘其产品价值提供了依据。This study compared the paclitaxel content from barks of Chinese yew with three different color fruit in Anhui Xuancheng. The sample solution was prepared under the condition： the ratio of raw material and chloroform was 1： 5, reflux exlracting for 2 h and decolorized by activated carbon. The content ofpaclitaxel was tested under 227 nm by HPLC method with Agilent HC-C18 column （250mm×4.6mm,5μm）, and take methanol-water-acetonitrile （24： 30： 36） as mobile phase. The paclitaxel content from barks of Chinese yew with red fruit is 0.000 564 mg/g, the yellow one is 0.000 785 mg/g, and the golden one is 0.000 703 mg/g. The result showed paclitaxel content in barks of Chinese yew with yellow and golden fruits were more than the one with red fruits, which initially set a clear medicinal value of the new Chinese yew species with yellow fruits and provides the basis for the further development of its products.

关 键 词：红豆杉 黄色果实 紫杉醇含量 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]