荧光光谱法研究富电子氨基酸与四苯乙烯-吡啶盐的相互作用  

Study on the Interaction between Rich Electronic Amino Acids and Tetraphenyletthene-pyridinium Salts by Fluorescent Spectrometry

作　　者：徐洲 梅高接 李亿保[1] 范小林[1] XU Zhou;MEI Gao-fie;LI Yi-bao;FAN Xiao-lin(Key Laboratory of Organo-pharmaeeutical Chemistry of Jiangxi Province,College of Chemistry and Chemical Engineering,Gannan Normal University,Ganzhou 341000,China)

机构地区：[1]赣南师范大学化学化工学院江西省有机药物化学重点实验室

出　　处：《化学试剂》2018年第11期1094-1097,共4页Chemical Reagents

基　　金：国家自然科学基金资助项目(21365003,21463003,51478123)

摘　　要：设计了一种新型生物分子传感材料水溶性四苯乙烯-吡啶盐（Tpp^4＋）分子。为了模拟生物环境,将溶液酸碱度调至pH 7. 4,用荧光光谱法研究了L-苯丙氨酸、L-色氨酸、L-异亮氨酸、L-组氨酸与Tpp^4＋分子的相互作用。研究结果发现,L-色氨酸对Tpp^4＋分子荧光有猝灭效应;根据不同温度下的Stern-Volmer曲线拟合得到,在T=298 K时,猝灭常数为355. 2L/mol,而在T=308 K时,猝灭常数为825. 4 L/mol;随着温度的升高,猝灭常数增大,由此判断猝灭机制为动态猝灭。A new biological molecular sensing materials was reported.In order to simulate the biological environment,the pH of the solution was adjusted to pH 7. 4,and the interaction between L-phenylalanine,L-tryptophan,L-isoleucine,L-histidine and Tpp^4＋ molecule was investigated by fluorescence spectroscopy.The results showed that L-Trp has a quenching effect on the molecular fluorescence of Tpp^4＋.Based on the Stern-Volmer curve fitting at different temperatures,the quenching constants were 355. 2 L/mol（ T= 298 K） and 825. 4 L/mol（ T= 308 K）,respectively.The quenching constants increased with the increase of temperature,and the quenching mechanism was dynamic quenching.

关 键 词：荧光光谱法 富电子氨基酸 荧光猝灭 四苯乙烯-吡啶盐 

分 类 号：O651[理学—分析化学]