泽泻提取物预处理对在体大鼠缺血再灌注损伤心功能及半胱氨酸蛋白酶-3的影响  

Effect of pretreatment with Rhizoma Alismatis extract on cardiac function and cysteinyl aspartate specific protease-3 in ischemia-reperfusion injury of rats

作　　者：吴小慧 刘菲 段忠心 王佳恒 Wu Xiaohui;Liu Fei;Duan Zhongxin;Wang Jiaheng(Department of Traditional Chinese Medicine,the Second Affiliated Hospital,University of South China,Hengyang 421001,China;Department of Anesthesiology,the second Affiliated Hospital,Oniversity of south China,Hengyang 421001,China)

机构地区：[1]南华大学附属第二医院中医科,衡阳421001 [2]南华大学附属第二医院麻醉科,衡阳421001

出　　处：《中华老年医学杂志》2018年第11期1292-1296,共5页Chinese Journal of Geriatrics

基　　金：衡阳市科技应用基础研究计划项目（2014KJ45）

摘　　要：目的观察泽泻提取物预处理对在体大鼠缺血再灌注损伤心功能及半胱氨酸蛋白酶-3（Caspase-3）的影响。方法随机将雄性SD大鼠分为假手术组（S组）、缺血再灌注组（IR组）、泽泻水提物组（S1组）、泽泻醇提物（S2组）及泽泻多糖组（S3组）。灌胃14 d后采用结扎冠状动脉左前降支制备心肌缺血-再灌注损伤模型，分别于平衡末（T1）、续灌末（T2）记录左室舒张末期内压（LVEDP）、左室收缩内压（LVSP）、左室内压最大上升/下降速率（±dp/dtmax）；测定肌酸激酶（CK）及乳酸脱氢酶（LDH）活性；T2时心肌梗死面积及caspase-3蛋白表达情况。结果T2与IR组LVEDP（6.70±0.22）mmHg、LVSP（86.16±15.11）mmHg、＋dp/dtmax（997.99±151.03）mmHg、－dp/dtmax（663.71±68.55）mmHg（1 mmHg＝0.133 kPa）、CK（10.54±2.04）U/L、LDH（296.51±7.00）U/L、心肌梗死面积（39.82±11.80）%及Caspase-3（123.42±14.77）比较，S1组、S2组及S3组LVEDP降低、LVSP及±dp/dtmax升高，LDH、CK及心肌梗死面积减少，caspase-3下调，〔（5.89±0.47）mmHg、（5.89±0.67）mmHg、（6.07±0.51）mmHg、（99.24±12.00）mmHg、（97.05±12.45）mmHg、（97.06 ±7.61）mmHg、（1 137.33±85.70）mmHg、（1 147.24±118.07）mmHg、（1 124.50±141.47）mmHg、（604.77±68.37）mmHg、（616.61±46.73）mmHg、（708.76±81.44）mmHg、（8.60±1.67）U/L、（8.90±1.27）U/L、（9.39±0.83）U/L、（239.33±30.81）U/L、（223.63±20.26）U/L、（241.19±45.56）U/L、（30.39±5.44）%、（32.18±5.90）%、（33.12±8.16）%、（73.44±15.28）（65.47±12.53）、（65.05±10.45），（均P〈0.01或〈0.05）〕。结论泽泻提取物预处理能有效改善在体大鼠缺血再灌注损伤心肌的心功能，使心肌梗死面积及心肌酶释放减少，其作用机制可能与下调心肌组织中凋亡蛋白Caspase-3的表达相关。ObjectiveTo observe the effect of pretreatment with Rhizoma Alismatis extract on cardiac function and cysteinyl aspartate specific protease-3（caspase-3）in ischemia-reperfusion injury of rats.MethodsMale Sprague-Dawley（SD）rats were randomized into a sham-operated group（S group）, an ischemia-reperfusion group（IR group）, Rhizoma Alismatis water extract group（S1 group）, Rhizoma Alismatis alcohol extract group（S2 group）and Rhizoma Alismatis polysaccharide group（S3 group）. At the end of 14-day of intragastric gavages, rats were subjected to 40 min（T1）of LAD（left anterior descending）coronary artery ligation（ischemia）and 120 min（T2）of ligation loosening（reperfusion）, called as myocardial ischemia-reperfusion（IR）models.Then, at the end of T1 and T2, the left ventricular end-diastolic pressure（LVEDP）, left ventricular systolic pressure（LVSP）, and maximum rise/fall rate of left intraventricular pressure（±dp/dtmax）were recorded respectively.The level of creatine kinase（CK）and lactate dehydrogenase（LDH）were determined.The area of myocardial infarction and the expression level of caspase-3 protein were tested.ResultsAt end of T2, compared with the index values of IR group as a non-treatment control[LVEDP（6.70±0.22）mmHg, LVSP（86.16±15.11）mmHg, ＋ dp/dtmax（997.99±151.03）mmHg, -dp/dtmax（663.71±68.55）mmHg, CK（10.54±2.04）U/L, LDH（296.51±7.00）U/L, the size of myocardial infarction（39.82±11.80）% and expression level of caspase-3（123.42±14.77）], the treatment groups of S1, S2, and S3 showed a lower levels of LVEDP[（5.89±0.47）mmHg, （5.89±0.67）mmHg, （6.07±0.51）mmHg], of activity of CK[（8.60±1.67）U/L（8.90±1.27）U/L, （9.39±0.83）U/L], of LDH[（239.33±30.81）U/L, （223.63±20.26）U/L, （241.19±45.56）U/L], of size of myocardial infarction[（30.39±5.44）%, （32.18±5.90）%, （33.12±8.16）%], and of expression level of caspase-3 protein[（73.44±15.28）, （65.47±12.53）, （65.05±

关 键 词：心肌再灌注损伤 泽泻 心脏功能 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]