保存期间试剂红细胞血型相关膜蛋白抗原性研究  

Study on antigenicity of blood group antigen related membrane protein of reagent RBCs during storage

作　　者：赵俸涌[1] 李勤[1] 郭忠慧[1] 张嘉敏[1] 刘李栋[1] 刘曦[1] 向东[1] 朱自严[1] ZHAO Fengyong;LI Qin;GUO Zhonghui;ZHANG Jiamin;LIU Lidong;LIU Xi;XIANG Dong;ZHU Ziyan(Shanghai Blood Center,Shanghai 200051.)

机构地区：[1]上海市血液中心

出　　处：《中国输血杂志》2018年第9期957-959,共3页Chinese Journal of Blood Transfusion

基　　金：上海市卫计委青年课题(20184Y0281);上海市卫计委课题(201640138);中国输血协会威高基金项目(CSBT-WG-2017-04)

摘　　要：目的研究红细胞膜蛋白抗原稳定性随保存时间变化。方法 D抗原、Fy^a抗原、Jk^a抗原、在保存节点（0、14、28、42 d）,对上述红细胞血型抗原抗原性进行检测。结果在标化后的抗体范围,使用国际标准抗体,应用流式细胞术,在42 d检出对比组之间的D抗原抗原性差异具统计学意义（下降11. 3%; P〈0. 05）; 42 d时,应用微柱凝胶卡,使用国际标准品（下降33%）、单克隆IgG-D（下降66%）、IgM-D（下降26%）,检出对比组之间的D抗原抗原性差异具统计学意义（P〈0. 01）,Fy^a抗原抗原性检出对比组之间的差异具统计学意义（下降33%,P〈0. 05）,Jk^a抗原抗原性未检出,对比组之间的差异具统计学意义。结论实验室应定期对谱细胞进行质控,对于弱抗体的检测宜使用较新鲜的谱细胞或新鲜细胞进行检测及确认。Objective To study the stability of erythrocyte membrane proteins and their antigenicity during storage.Methods Rh blood group system D antigen; Duffy blood group system Fy^a antigen; Kidd blood group system Jk^a antigen were selected. Flow cytometry and serological gel method were used to detect the blood group antigen. Results Using the calibrated antibody,with the British Standard for Anti-D（ Rho） antibodies,significant differences were detected by flow cytometry at day 42（ down 11. 3%; P〈0. 05）. Gel cards also detected significant changes in the standard（ 33% decrease; P〈0. 01）,the monoclonal IgG-D（ down 66%） and the IgM-D（ down 26%） samples. Fy^a antigen were detected using IgG-Fy^a by microcolumn gel,at day 42 the difference was significant（ down 33%,P〈0. 05）. Jk^a antigen was detected by IgM-Jk^a,the difference was,however,not significant. Conclusion In the immunohematology reference laboratory and transfusion department,the quality of the reagent cells should be regularly controlled. For the detection of weak antibodies,fresher reagent cells or fresh cells should be used for detection and confirmation.

关 键 词：试剂红细胞保存 红细胞抗原 抗原性 质量控制 

分 类 号：R457.11[医药卫生—治疗学]