Effect of Torsion on Cisplatin-Induced DNA Condensation  

作　　者：Bo Li Chao Ji Xi-Ming Lu Yu-Ru Liu Wei Li Shuo-Xing Dou Hui Li Peng-Ye Wang 李波;季超;吕袭明;刘玉如;李伟;窦硕星;李辉;王鹏业(National Laboratory for Condensed Matter Physics and Key Laboratory of Soft Matter Physics, Institute of Physics,Chinese Academy of Sciences;School of Physical Sciences, University of Chinese Academy of Sciences;School of Bioscience and Biotechnology, Weifang Medical University)

机构地区：[1]National Laboratory for Condensed Matter Physics and Key Laboratory of Soft Matter Physics,Institute of Physics,Chinese Academy of Sciences,Beijing 100190 [2]School of Physical Sciences,University of Chinese Academy of Sciences,Beijing 100049 [3]School of Bioscience and Biotechnology,Weifang Medical University,Weifang 261053

出　　处：《Chinese Physics Letters》2018年第11期90-94,共5页中国物理快报（英文版）

基　　金：Supported by the National Natural Science Foundation of China under Grant Nos 11674383,11774407 and 11874415;the Key Research Program of Frontier Sciences of Chinese Academy of Sciences under Grant No QYZDB-SSW-SLH045;the National Key Research and Development Program under Grant No 2016YFA0301500;the Higher Educational Science and Technology Program of Shandong Province under Grant No J16LM06;the Natural Science Foundation of Shandong Province under Grant No ZR2014AQ025;the Program of Shandong Provincial Government International Exchanges

摘　　要：We investigate the effect of torsion on DNA condensation with the covalently closed circular DNA as the torsionconstrained system, using an atomic force microscope. It is found that there are two stages in the DNA condensation process under torsional constraint. At the early stage, the low torsion will accelerate DNA condensation by promoting the formation of micro-loops or intersection structures; while at the later stage, the increasing torsion will slow it down by preventing the crosslinking of cisplatin and DNA since the DNA molecule becomes more rigid. Our results show the important role of torsion in DNA condensation and sheds new light on the mechanism for DNA condensation.We investigate the effect of torsion on DNA condensation with the covalently closed circular DNA as the torsionconstrained system, using an atomic force microscope. It is found that there are two stages in the DNA condensation process under torsional constraint. At the early stage, the low torsion will accelerate DNA condensation by promoting the formation of micro-loops or intersection structures; while at the later stage, the increasing torsion will slow it down by preventing the crosslinking of cisplatin and DNA since the DNA molecule becomes more rigid. Our results show the important role of torsion in DNA condensation and sheds new light on the mechanism for DNA condensation.

关 键 词：cccDNA Effect of Torsion on Cisplatin-Induced DNA Condensation 

分 类 号：Q523[生物学—生物化学]