不同性别大白猪肌肉全基因组高分辨率单碱基甲基化差异分析  

High Resolution and Single Base Genome-wide Methylation Variance Analysis of Muscle of Large White Pigs with Different Sexes

作　　者：郭添福[1] 张志燕[1] 陈冬 姚天雄 肖石军[1] 黄路生[1] GUO Tian-fu;ZHANG Zhi yan;CHEN Dong;YAO Tian xiong;XIAO Shi jun;HUANG Lu sheng(State Key Laboratory for Pig Genetic Improvement and Production Technology,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学省部共建猪遗传改良与养殖技术国家重点实验室,南昌330045

出　　处：《畜牧兽医学报》2018年第11期2326-2339,共14页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31760656);广东扬帆计划引进创新创业团队项目(2016YT03H062)

摘　　要：旨在通过不同性别大白猪的全基因组高分辨率单碱基甲基化差异分析,检测差异甲基化区域(DMR)和差异甲基化基因(DMG),为进一步解析公母猪骨骼肌发育差异奠定基础。本研究采用全基因组重亚硫酸盐测序(WGBS)技术分析了4头210日龄长白猪(公、母各半)背最长肌全基因组DNA的甲基化程度和差异甲基化区域,探讨性别间DNA甲基化水平的差异。结果表明,全基因组范围约有5%的胞嘧啶(C)发生了甲基化(mC);母猪和公猪的总体甲基化水平基本一致。共检测到1 629个DMRs和841个DMGs。母猪的DMRs平均甲基化水平明显高于公猪。通过基因本体分析(GO)和相关信号通路(KEGG)分析,共检测到171个GO条目和10个信号通路,显著富集在轴突导向、细胞连接、细胞粘附、ECM受体互作等相关过程中;筛选出5个与不同性别肌肉调节相关的候选基因。本研究绘制了不同性别大白猪的高分辨率单碱基全基因组甲基化图谱,为不同性别表观遗传研究以及肌肉发育和肉质相关候选基因的筛选提供了信息参考。The aims of this study were to detect the differentially methylated region （DMR） and differentially methylated gene （DMG） and lay a foundation for further analyzing the skeletal mus cle development difference between male and female pigs by high resolution and single base genome wide methylation variance analysis for Large White pigs of different genders. In this study, four 210 day old Large White pigs （two males and two females） were scanned by the whole genome bisulfite sequencing method （WGBS）. The degree of methylation and differentially methylated region in the whole genome DNA of longissimus dorsi （LD） muscle were studied to explore the difference of DNA methylation level between male and female pigs. The results showed that about 5% Cytosine （C） were methylated in the whole genome. The overall methylation level of sows and boars was basically the same. A total of 1629 DMRs and 841 related DMGs were detec ted. The DMRs methylation level of sows was higher than that of boars. One hundred and seventy one GO terms and 10 related signal pathways were detected by GO and KEGG analysis, respectively, which were showed to be significantly enriched in cell junction, axon guidance, celladhesion molecule binding, ECM receptor interaction, and so on. Five candidate genes werescreened out in muscle tissues of male and female pigs. This study provided the single base and highresolution genomewide methylation pattern of male and female Large White pigs. The results providereference information for the epigenetic study of different genders and screening candidate genes related tomuscle development and meat quality.

关 键 词：性别 肌肉 全基因组 甲基化 

分 类 号：S828.2[农业科学—畜牧学]