基于线粒体12S基因全序列分析我国绵羊痒螨(兔亚种)的遗传多样性  

Genetic Diversity of Psoroptes ovis var. cuniculi Determined by the Full-length of Mitochondrial 12S Gene in China

作　　者：李燕方 古江[1] 袁媛 谢跃[1] 赖为民[1] 杨光友[1] 古小彬[1] LI Yan fang;GU Jiang;YUAN Yuan;XIE Yue;LAI Wei rain;YANG Guang you;GU Xiao bins(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学动物医学院,成都611130

出　　处：《畜牧兽医学报》2018年第11期2468-2476,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金青年基金项目(31402188);国家级大学生创新训练计划项目(201610626017)

摘　　要：为了探讨我国绵羊痒螨(兔亚种)的遗传变异情况及群体遗传结构,采用PCR测序技术测定我国5个地区(华北、华东、华中、西北、西南)绵羊痒螨(兔亚种)线粒体12S基因的全序列,并进行遗传多样性分析。结果共获得89条序列,长度均为657bp,核苷酸变异位点76个,单倍型50个(H1～H50),单倍型多样性(Hd)与核苷酸多样性(π)分别为0.931 05和0.005 59。进一步分析发现5个地理种群遗传分化程度较弱(Fst=0.042 322),基因交流非常频繁(Nm=5.657 372)。中性检验结果均为负值(Tajima’s D=-0.928 31,Fu’s Fs=-7.066 71),并且差异性不显著(P<0.05)。构建的NJ树和单倍型网络图均显示绵羊痒螨(兔亚种)5个地理种群的50个单倍型散在分布于不同的支系内,未形成明显的地理分布格局。我国绵羊痒螨(兔亚种)具有较高的遗传多样性,且有一定的遗传分化,基因交流频繁,但未形成以兔品种、温度带和地域来源划分的种群遗传结构。To explore the genetic diversity and genetic structure of Psoroptes owis vat. cuniculi, the complete sequence of mitochondrial 12S gene of P. ovis vat. cuniculi isolates from 5 geo graphical regions （Huabei, Huadong, Huazhong, Xibei, and Xinan） in China, were obtained by PCR sequencing techniques, and evaluated the genetic diversity of P. ovis population. Eighty nine sequences of mitochondrial 12S were obtained. The multiple alignments of the full 12S genefrom 89 isolates were 657 bp. These sequences contained 76 variable sites and were classified into50 haplotypes （H1 HS0）. The values of haplotypes diversitiy （Hd） and nucleotide diversity （π） were 0. 931 05 and 0. 005 59 respectively. There were low level of genetic differentiation, buthigh level of gene flow （Fs=0. 042 322, Ns=-5. 657 372） among the 5 geographical population.In P. ovis population, the average values of Tajima＇s D and Fu＇s Fs were negative （Tajima＇s D 0.928 31, Fu＇sFs=7.066 71） and there was no significant difference （P〈0.05）. NJ phylogenetic tree and haplotype network were both revealed that 50 haplotypes from 5 different geographical regions dispersed across different clades without associating with the geographical locations. The results revealed that there was a high level of genetic diversity , little populationgenetic differentiation and high gene flow in the P. ovis var. cuniculi population of China, but population structure has not formed according to rabbits breed, temperature zones and geography ori

关 键 词：绵羊痒螨(兔亚种) 线粒体12S基因 遗传多样性 

分 类 号：S852.746[农业科学—基础兽医学]