玛咖酰胺对小鼠阴茎海绵体平滑肌细胞eNOS基因表达的影响及机制  

Effect of Macamide on Expression of eNOS Gene in Penis Cavernosum Smooth Muscle Cells of Mice

作　　者：李宝鑫 谢正媛 郑水 袁彦玲 LI Bao-xin;XIE Zheng-yuan;ZHENG Shui;YUAN Yan-ling(Yunnan Population and Family Planning Research Institute,Kunming 650021,China)

机构地区：[1]云南省人口和计划生育科学技术研究所,昆明650021

出　　处：《国际生殖健康／计划生育杂志》2018年第6期445-449,共5页Journal of International Reproductive Health/Family Planning

基　　金：云南省科技计划项目青年项目(2017FD031)

摘　　要：目的:探讨玛咖酰胺对小鼠阴茎海绵体平滑肌细胞(PCSMC)中内皮型一氧化氮合酶(eNOS)表达量的影响。方法:将玛咖酰胺溶液作用于小鼠PCSMC后检测其中eNOS的表达量及蛋白激酶B(AKT)、c-Jun氨基末端激酶(JNK)、P38/丝裂原活化蛋白激酶(P38/MAPK)的蛋白表达水平的变化。然后将玛咖酰胺处理过的小鼠PCSMC加入了P38的抑制剂SB203580,检测P38及eNOS蛋白表达水平的变化。结果:玛咖酰胺溶液作用于小鼠PCSMC后,PCSMC中eNOS及P38/MAPK蛋白表达水平均明显升高,AKT、JNK蛋白表达水平无明显改变。加入P38的抑制剂SB203580后,玛咖酰胺处理过的小鼠PCSMC中P38及eNOS蛋白表达水平均明显降低。P38的抑制剂SB203580能够明显逆转玛咖酰胺诱导的eNOS上调。结论:玛咖酰胺可通过活化小鼠PCSMC中的P38信号,促进eNOS的生成。Objective：To explore the effect of macamide, an effective extract of maca, on the expression of eNOS in penis cavernosum smooth muscle cells （PCSMC）. Methods：The cultured PCSMC was treated with macaamide （100 nmol/L, 100 μmol/L, 100 mmol/L） for 24 h. The expressions of eNOS mRNA and AKT, JNK, P38/MAPK proteins were tested. The PCSMC was also treated with macaamide and SB203580, the inhibitor of P38. The expressions of P38 and eNOS proteins were then tested. Results：After the treatment with macaamide, the expressions of eNOS mRNA and P38/MAPK proteins in PCSMC were significantly increased while the expressions of AKT and JNK proteins were not significantly changed. The expressions of P38 and eNOS proteins were significantly decreased in the PCSMC treated with both macaamide and SB203580, suggesting that SB203580 reversed the upregulation of eNOS induced by macaamide. Conclusions：Macamide can promote the expression of eNOS in PCSMC by activating the P38 pathway.

关 键 词：一氧化氮合酶 阴茎 阴茎勃起 肌细胞 平滑肌 玛咖酰胺 阴茎组织海绵体平滑肌细胞 

分 类 号：R698[医药卫生—泌尿科学]