红花龙胆对肺炎链球菌肺炎防治作用  

Prevention and treatment effect of Gentiana rhodantha on Streptococcus pneumoniae pneumonia in mice

作　　者：王飞清 陶奕汐[1] 李学会[1] 何秀丽[1] 杨剑 刘燕青[1] 李丽[1] 罗君[1] WANG Fei-qing;TAO Yi-xi;LI Xue-hui(Department of Clinical Laboratory,The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou Province 550001,China)

机构地区：[1]贵阳中医学院第一附属医院,贵州贵阳550001

出　　处：《中国公共卫生》2018年第11期1484-1486,共3页Chinese Journal of Public Health

基　　金：贵州省科技计划项目(黔科合支撑[2018]2772);贵州省科技合作计划项目(黔科合LH字[2015]7801号);贵州省科技计划项目(黔科合LH字[2017]7147号);贵州省中医药管理局中医药;民族医药科学技术研究课题(QZYY–2016–099);苗族医学研究协同创新中心项目(黔教合协同创新字[2015]05);贵阳中医学院科研项目(贵中医科院内[2016]55号)

摘　　要：目的探讨苗药红花龙胆对肺炎链球菌肺炎的防治作用。方法 30只清洁级昆明小鼠随机分为对照组、模型组和红花龙胆组3组,每组10只;在小鼠感染后出现闭眼、呼吸急促等异常情况时,10%水合氯醛麻醉后眼球取血法处死小鼠,分离血清,采用酶联免疫法检测血清C–反应蛋白(CRP)、降钙素原(PCT)、白细胞介素6(IL-6)和白细胞介素8(IL-8)水平,光镜下观察肺组织病理形态学改变。结果与对照组比较,模型组和红花龙胆组小鼠CRP [(27.28±7.44)和(20.17±3.91)μg/mL]、PCT [(8.12±1.90)和(5.29±1.28)ng/mL]、IL-6[(256.40±76.45)和(174.14±30.60)pg/mL]、IL-8[(134.71±33.21)和(84.40±14.94)pg/mL]水平均升高,差异均有统计学意义(均P <0.01);与模型组比较,红花龙胆组小鼠CRP、PCT、IL-6、IL-8水平均低于模型组,差异均有统计学意义(均P <0.01)。对照组小鼠气管上皮纤毛整齐排列,肺泡腔内无炎性分泌物,肺泡结构完好;模型组小鼠肺组织病理改变明显,支气管管腔狭窄,充满炎性渗出物,肺泡腔内炎性细胞浸润;红花龙胆组小鼠肺组织与模型组比较,炎性渗出物明显减少,炎症明显减轻。结论红花龙胆对肺炎链球菌肺炎具有防治作用。Objective To study prevention and treatment effect of Gentiana rhodantha （GR） on Streptococcus pneumoniae （Sp） pneumonia in mice. Methods Thirty clean Kunming mice were randomly divided into 3 groups （10 in each group）： a control, a model, and a GR group. Mouse Sp pneumonia model was established with intranasal instillation of bacteria solution. Mice in GR group were administered with GR decoction by intragastric gavage. Eyeball blood samples of all the mice were collected after 10% chloral hydrate anesthesia when there were eye closure and tachypnea in Sp infected mice. Serum levels of C-reaction protein （CRP）, procalcitonin （PCT）, interleukin-6 （IL-6）, and interleukin-8 （IL-8） were detected with enzyme-linked immunosorbent assay; pathological changes of lung tissues were observed under light microscope. Results Significantly increased CRP （27.28 ± 7.44 ug/ml, 20.17 ± 3.91 ug/ml）, PCT （8.12 ± 1.90 ng/ml, 5.29 ± 1.28 ng/ml）, IL-6 （256.40 ± 76.45 pg/ml, 174.14 ± 30.60 pg/ml）, and IL-8 （134.71 ± 33.21 pg/ml, 84.40± 14.94 pg/ml） were detected in the mice of model and GR group compared to those in the mice of the control group （all P 〈 0.01）; while the levels of CRP, PCT, IL-6, and IL-8 of the mice of GR group were significantly lower than those of the mice in the model group （P 〈 0.01 for all）. Neatly arranged tracheal epithelium cilium and intact alveolar structure were observed but no inflammatory secretion in alveolar spaces was found in the mice of control group; whereas, obvious pathological changes were discovered in the mice of model group, including narrowed bronchial cavities, large amount of inflammatory secretion and inflammatory cell infiltration in alveolar spaces. Compared to those in the mice of model group, decreased amount of inflammatory secretion and alleviated inflammation were discovered in the mice of GR group. Conclusion Gentiana rhodantha could prevent lung inflammation caused by Streptococcus pneumoniae in mice.

关 键 词：红花龙胆 肺炎链球菌肺炎 防治作用 

分 类 号：R378.12[医药卫生—病原生物学] R932[医药卫生—基础医学]