马尾松针提取物对镉毒16HBE细胞保护作用  

Protective effect of Pinus massoniana pine needle extract against cadmium chloride-induced cytotoxicity in 16HBE cells

作　　者：袁琛 练玉银 姜志杰 任亮 周珊珊 雷毅雄 YUAN Chen;LIAN Yu-yin;JIANG Zhi-jie(Department of Preventive Medicine,School of Public Health,Guangzhou Medical University,Guangzhou,Guangdong Province 510182,China)

机构地区：[1]广州医科大学公共卫生学院预防医学系,广东广州510182

出　　处：《中国公共卫生》2018年第11期1516-1519,共4页Chinese Journal of Public Health

基　　金：国家自然科学基金(81573177;81373038);广州市教育科学"十二五"规划2015课题(12101533519)

摘　　要：目的研究马尾松松针提取液对氯化镉(CdCl2)致16HBE细胞毒性的保护作用。方法用萃取法提取马尾松松针乙醇提取物(PMNE),分别用1、100和500μg/mL的PMNE(低、中、高浓度组)预处理16HBE细胞6 h,随后用25μmol/L CdCl2毒染至48 h,同时设阴性对照组(正常培养16HBE细胞)及空白对照组(无细胞组),用CCK8法检测细胞相对生存率,硫代巴比妥酸(TBA)法检测各组细胞内丙二醛(MDA)含量,qPCR法检测各组细胞凋亡相关基因Bcl-2及Bax相对表达量。结果与阴性对照组相比,CdCl2单独作用组细胞相对生存率下降(P <0.05),细胞内MDA表达量升高(P <0.05);低、中、高浓度PMNE预处理组细胞与CdCl2单独处理组相比,细胞相对生存率均升高(P <0.05),MDA含量均降低(P <0.05),且浓度越高细胞相对生存率越高(P <0.05),MDA含量越低(P <0.05)。与CdCl2单独作用组相比,低、中、高浓度PMNE预处理组抑凋亡基因Bcl-2相对表达量均上调(P <0.05),促凋亡基因Bax相对表达量均下调(P <0.05),且浓度越高Bcl-2相对表达量越高(P <0.05)。结论 PMNE对CdCl2所致的细胞毒性有一定程度的保护作用。Objective To study the protective effect of Pinus massoniana pine needle extract （PMNE） on cadmium chloride-induced cytotoxicity in human bronchial epithelial cells （16HBE）. Methods Ethanol extraction method was used to prepare PMNE. The 16HBE cells of low-, moderate-, and high-dose groups were treated with 1, 100, and 500 μg/mL PMNE for 6 hours; then the 16HBE cells were exposed to 25 μmol/L cadmium chloride （CdC12） for 48 hours. A negative control group （normally cultured 16 HBE cells） and a blank control group （culture medium only） were also established. Cell Counting Kit-8 （CCK8） method was used to detect relative survival rate of the cells. Thiobarbituric acid （TBA） method was adopted to measure intracellular content of malondialdehyde （MDA）. Quantitative PCR （qPCR） was applied to determine relative cellular expressions of apoptosis-related genes of B cell lymphoma 2 （Bcl-2） and Bcl-2-associated X protein. Results Compared with that of negative control ceils, the relative survival rate of cells treated with CdC12 alone was decreased significantly （P 〈 0.05） but the intracellular MDA expression was increased （P 〈 0.05）. The relative survival rate of the cells treated with low-, moderate-, and high-dose PMNE were increased but the intracellular MDA of the cells was decreased, both in a significant dose-response manner, in comparison with those of the cells treated with CdCI2 alone significantly （P 〈 0.05 for all）. Significantly increased relative expression of anti-apoptotic gene Bcl-2 and decreased relative expression of pro-apoptotic gene Bax, both in a significant dose-response manner, were observed in the cells with low-, moderate, and high-dose PMNE treatment in contrast to those of the cells treated with CdCI2 alone （all P 〈 0.05）. Conclusion Ethanol extract of Pinus massoniana pine needles has a certain protective effect against chloride-induced cytotoxicity in human bronchial epithelial cells in vitro.

关 键 词：马尾松 松针提取液 16HBE细胞 氯化镉(CdCl2) 

分 类 号：R994.6[医药卫生—毒理学]