独脚金水溶性多糖的结构表征及其清除自由基活性  

Structural Characterization and Its Free Radical Effect of a Water-soluble Polysaccharide from Strigaasiatica (L.)O.Ktze.

作　　者：王坤[1] 阿西娜[1] 李默 刘春兰[1,2] WANG Kun;A-XI Na;LI Mo;LIU Chun-lan(College of Life and Environmental Science,Minzu University of China,Beijing 100081,China;Beijing Engineering Research Center of Food Environment and Public Health,Beijing 100081,China)

机构地区：[1]中央民族大学生命与环境科学学院,北京100081 [2]北京市食品环境与健康工程技术研究中心,北京100081

出　　处：《高分子通报》2018年第11期52-59,共8页Polymer Bulletin

基　　金：中央民族大学一流大学一流学科建设项目(YLDX01013);"111创新引智计划"工程项目(B08044);准格尔北部生态保育技术集成与示范(2014BAC15B04)

摘　　要：独脚金粗多糖(Strigaasiatica(L.)O.Ktze. polysaccharide,SKPc)经DEAE-Sephadex A-25以及Sephadex G-100柱层析分离纯化后得到独脚金多糖组分(SKP~*_1)。采用高效液相色谱(HPLC)法检测SKP~*_1,表明多糖SKP~*_1极性、相对分子质量是均一的,且其相对分子质量为14852u。采用气相色谱(GC)分析法研究SKP~*_1的单糖组成为葡萄糖(Glu)、阿拉伯糖(Ara)、半乳糖(Gal)、木糖(Xyl)、甘露糖(Man)、鼠李糖(Rha),相对摩尔比是2.5∶2.0∶0.85∶0.4∶0.5∶1.0;可能主要以β型糖苷键连接。采用化学分析和仪器分析结合进行SKP~*_1结构分析,表明SKP~*_1结构是Ara以β-(1→3)糖苷键连接构成主链之一,2,4或2,5位有支链;Gal以β-(1→6)糖苷键连接构成SKP~*_1的主链之一,且在3位上有分枝。支链由β-(1→4或1→5)Xyl、β-(1→4,1→6,1→2)Glc构成。Rha、Ara、Man、Gal、Glc都有一部分构成糖分子的末端残基。体外清除羟基实验结果表明,SKP~*_1有较好地清除羟基自由基的活性。Abstraet.-SKPe was extracted from Strigaasiatica （L.）O. Ktze. SKPe which was deproteinized and decolorized was isolated and purified using DEAE-Sephadex A-25 and Sephadex G-100 and got SKP1. High performance liquid chromatography （HPLC） indicated that SKP1 was a homogeneity polysaccharide, and the average molecular weight of SKP1 was approximately 14852 u. The water-soluble polysaccharide SKP1 has been analyzed by GC, it was composed of Glu, Ara, Gal, Xyl, Man, Rha, with a molar ratio of 2.5.＇2.0z0.85：0.4：0. 5.＇1.0. The most of the sugar residues were existed as/}-configuration. And the main chain was made up of β-（→3）Ara, and β-（1→6）Gal, on average. The side chain was composed of β-（1→4 or 1→5）-Rha and （1→6, 1→4, 1→2）-Glc. The terminal residue was composed of Ara, Man, Gal and Glc. The scavenging rates of the SKP1 on hydroxyl was good.

关 键 词：独脚金 多糖 分离纯化 结构表征 自由基 

分 类 号：O629[理学—有机化学]