In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum  

作　　者：Bruno Jose Martins Da Silva Sandro Wilson Gomes Pereira Ana Paula Drummond Rodrigues Jose Luiz Martins Do Nascimento Edilene Oliveira Silva 

机构地区：[1]Laboratory of Structural Biology,Institute of Biological Sciences,Federal University of Pard,Belem,Pard 66075-110,Brazil [2]National Institute of Science and Technology in Structural Biology and Bioimaging(INCT-INBEB),Rio de Janeiro 21941-901,Brazil [3]Laboratory of Electron Microscopy,Department of Health Surveillance,Ministry of Health,Evandro Chagas Institute,Belem,Pard 66087-082,Brazil [4]Laboratory of Molecular and Cellular Neurochemistry,Institute of Biological Sciences,Federal University of Pard,Belem,Pard 66075-110,Brazil [5]National Institute of Science and Technology in Neuroimmunomodulation(INCT-NIM),Rio de Janeiro 21040-360,Brazil

出　　处：《Journal of Integrative Medicine》2018年第6期404-410,共7页结合医学学报（英文版）

基　　金：supported by the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico(CNPq-grant number 424820/2016-1);Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior(CAPES);Edital PAPQ PROPESP-UFPA;the Instituto Nacional de Biologia Estrutural e Bioimagem-INBEB(CNPq-grant number 465395/2014)

摘　　要：Objective： In the present study, we evaluated the effects of the aqueous extract of Physalis angulata root （AEPa） on Leishmania infanturn proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages. Methods： I., infantum promastigotes were treated with 50 and 100 μg/mL AEPa for 72 h and then antipro- mastigote assay was performed by counts in a Newbauer chamber, morphological changes were analyzed by transmission electron microscopy and the mechanism of the leishmanicidal activity was detected. In addition, macrophages were infected with L. infantum and were used to evaluate anti-amastigote activity of AEPa and effects of AEPa on cytokine secretion after 72-hour treatment. Results： Treatment with AEPa reduced the numbers ofL infantum promastigotes （50% inhibitory concen- tration （ICso） = 65.9μg/mL; selectivity index （SI）= 22.1） and amastigotes （ICso = 37.9 μg/mL; SI = 38.5） compared with the untreated control. Amphotericin B reduced 100% of the promastigote numbers after 72 h of treatment （IC50 = 0.2μg/mL）. AEPa induced several morphological changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72 h. AEPa （100 μg/mL） promoted tumor necrosis factor-α secretion in macrophages infected with L. infantum after 72 h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEPa showed no cytotoxic effect on J774-A1 cells （50% cytotoxic concentration 〉1000μg/mL）. Conclusion： AEPa presented antileishmanial activity against the promastigotes and amastigotes of I.. infantum without macrophage cytotoxicity; these results show that natural products such as P. angulata have leishmanicidal potential and in the future may be an alternative treatment for leishmaniasis.Objective： In the present study, we evaluated the effects of the aqueous extract of Physalis angulata root （AEPa） on Leishmania infanturn proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages. Methods： I., infantum promastigotes were treated with 50 and 100 μg/mL AEPa for 72 h and then antipro- mastigote assay was performed by counts in a Newbauer chamber, morphological changes were analyzed by transmission electron microscopy and the mechanism of the leishmanicidal activity was detected. In addition, macrophages were infected with L. infantum and were used to evaluate anti-amastigote activity of AEPa and effects of AEPa on cytokine secretion after 72-hour treatment. Results： Treatment with AEPa reduced the numbers ofL infantum promastigotes （50% inhibitory concen- tration （ICso） = 65.9μg/mL; selectivity index （SI）= 22.1） and amastigotes （ICso = 37.9 μg/mL; SI = 38.5） compared with the untreated control. Amphotericin B reduced 100% of the promastigote numbers after 72 h of treatment （IC50 = 0.2μg/mL）. AEPa induced several morphological changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72 h. AEPa （100 μg/mL） promoted tumor necrosis factor-α secretion in macrophages infected with L. infantum after 72 h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEPa showed no cytotoxic effect on J774-A1 cells （50% cytotoxic concentration 〉1000μg/mL）. Conclusion： AEPa presented antileishmanial activity against the promastigotes and amastigotes of I.. infantum without macrophage cytotoxicity; these results show that natural products such as P. angulata have leishmanicidal potential and in the future may be an alternative treatment for leishmaniasis.

关 键 词：Leishmanicidal activity Leishmania infantum Reactive oxygen species APOPTOSIS Physalis angulata 

分 类 号：TU991.21[建筑科学—市政工程] Q26[生物学—细胞生物学]