虎杖基因PcMYB1真核表达载体构建及遗传转化  

Construction of Eukaryotic Expression Vector of Polygonum cuspidatum Gene PcMYB1 and Its Genetic Transformation

作　　者：李晓筱 刘婵 徐俊雄 王岩岩[1] 伍翔 覃建兵[1] 柳忠玉[1] 

机构地区：[1]长江大学生命科学学院,湖北荆州434025

出　　处：《长江大学学报（自然科学版）》2018年第22期39-42,共4页Journal of Yangtze University(Natural Science Edition)

基　　金：国家级大学生创新创业项目(201810489013);长江大学湿地生态与农业利用教育部工程研究中心开放基金项目(KF201511)

摘　　要：在虎杖(Polygonum cuspidatum Sieb.et Zucc.)叶片中获得了1个R2R3-MYB转录因子基因,命名为PcMYB1。推测其在苯丙烷代谢中起负调控作用。为了探索PcMYB1的的功能,构建了PcMYB1基因的真核过量表达载体,通过农杆菌介导,利用花絮浸染法转化拟南芥,转化效率为0.11%。经潮霉素筛选和PCR鉴定,结果表明PcMYB1成功转入拟南芥中。One R2R3-MYB transcription factor named PcMYB1 was isolated from the leaf of Polygonum cuspidatum.It was speculated that it played a negative regulatory role in phenylpropanoid metabolism.In order to evaluate the function of PcMYB1,the plant expression vector pCAMBIA-35 SPcMYB1 was constructed and introduced into Arabidopsis thalianathrough Agrobacterium tumefaciens by means of the floral dip method.The transformants were screened on the medium containing hygromycin and the integration of PcMYB1 gene into the transgenic plants was verified via the PCR.The results showed that the transformation efficiency was 0.11% and PcMYB1 was successfully transferred into Arabidopsis thaliana.

关 键 词：虎杖(Polygonum cuspidatum Sieb.et Zucc.) MYB转录因子 载体构建 拟南芥转化 

分 类 号：Q789[生物学—分子生物学]