慢性阻塞性肺疾病的早期诊断生物标志物初筛  

Preliminary screening of biomarkers for early diagnosis of chronic obstructive pulmonary disease

作　　者：严琼[1] 黄忠辉 朱卫华[1] 左斌[1] 蓝芬[1] 陈子谓[1] 王钊逸 张艳利[3,5] 王新明 YAN Qiong;HUANG Zhonghui;ZHU Weihua;ZUO Bin;LAN Fen;CHEN Ziwei;WANG Zhaoyi;ZHANG Yanli;WANG Xinming(Department of Respiratory Diseases,Guangzhou No.12 People s Hospital,Guangzhou 510620,Guangdong,China)

机构地区：[1]广州市第十二人民医院呼吸内科,广东广州510620 [2]环境保护部华南环境科学研究所国家环境保护环境污染健康风险评价重点实验室广东省水与大气污染防治重点实验室 [3]中国科学院广州地球化学研究所有机地球化学国家重点实验室广东省环境资源利用与保护重点实验室 [4]中国科学院大学 [5]中国科学院城市环境研究所中国科学院区域大气环境研究卓越创新中心

出　　处：《中南医学科学杂志》2018年第6期616-620,共5页Medical Science Journal of Central South China

基　　金：国家自然科学基金委员会(编号:41571130031);广东省自然科学基金委员会(编号:2016A030313164);广州市卫生和计划生育委员会(编号:20161A010050)

摘　　要：快速准确的早期诊断技术是有效防治慢性阻塞性肺疾病(COPD)、改善患者生命质量、延长其寿命、降低死亡和减少医疗费用的重要途径,也是当前医学研究中重要前沿科学问题。本研究基于患者呼出气中挥发性有机物携带大量的生理、病理信息,利用质子转移反应-飞行时间质谱仪,分析了15例患者和50例健康对照组的呼吸气中挥发性有机物的组分及浓度。初步筛选出18种COPD患者的特征性呼气化合物,其中5种化合物与前人的研究一致,可作为生物标志物应用于COPD的早期诊断。另外13种新发现的化合物,具备作为生物标志物应用于COPD早期诊断的前景,但仍需进一步的研究以证实。本研究丰富了COPD生物标志物库,对于建立非侵入性、快捷的COPD早期诊断方法具有重要支撑作用。The techniques for fast, accurate and early diagnosis of chronic obstructive pulmonary disease （COPD） are the important access to effective prevention and cure of COPD, improving the quality of their life, extending their life span, decreasing their mortality and reduction in the treatment costs. Also they are the leading-edge issues in current medicine study in the world. In the present study, based on the large amount of physio-pathological information included in the volatile organic compounds （VOCs） from the exhaled air of the patients, the proton transfer reaction time-of-flight mass spectrometry was employed to determine the components and concentrations of VOCs in inhaled and exhaled air from 15 patients of COPD and 50 healthy controls. The results showed that 5 compounds, out of the 18 compounds which were preliminarily screened as the distinctive components of the exhaled air from COPD in the present study, are consent with the results of other research, and might be used as biomarkers for the early diagnosis of COPD. Additionally, the 13 novel compounds are perspective biomarkers for the early diagnosis of COPD, but needed further deep investigation. The data of this study had enriched the biomarkers for COPD and will take an important role in the establishment of the fast-and-noninvasive early diagnosis of COPD in the future.

关 键 词：慢性阻塞性肺疾病 呼吸气分析 挥发性有机物 生物标志物 质子转移反应-飞行时间质谱仪 

分 类 号：R563[医药卫生—呼吸系统]