益气活血化瘀汤对膜性肾病大鼠肾脏组织Podocin表达的影响  

Effects of Yiqi Huoxue Huayu Decoction on Expression of Podocin in Rats with Membranous Nephropathy

作　　者：陈玲玲[1] 左扬 冯源 程锦国[1] Chen Lingling;Zuo Yang;Feng Yuan(Wenzhou Hospital of Traditional Chinese Medicine Affiliated to Zhejiang University of Traditional Chinese Medicine,Zhejiang,Wenzhou 325000,China)

机构地区：[1]浙江中医药大学附属温州市中医院,浙江温州325000 [2]温州医科大学,浙江温州325000

出　　处：《中国中医急症》2018年第11期1912-1916,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：2018年浙江省中医药重大研究项目(2018ZY011)

摘　　要：目的观察益气活血化瘀汤对膜性肾病大鼠肾脏组织Podocin蛋白表达的影响,为临床治疗膜性肾病(MN)提供依据。方法 SD大鼠随机分为正常对照组和造模组,其中造模组采用抗Fx1A血清一次性尾静脉注射诱导被动型Heymann肾炎模型,造模成功后将造模组随机分为模型组、洛丁新(盐酸贝那普利)组、益气活血化瘀汤低、中、高剂量组。各组按相应剂量灌胃4周,每周留取大鼠24 h尿量,检测24 h尿蛋白定量(UTP)。于4周末处死大鼠,心脏取血,检测血肌酐(Scr)、尿素氮(BUN)、血清总蛋白(TP)、血清白蛋白(ALB)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、三酰甘油(TAG),取肾脏组织行Masson、PASM染色观察肾脏组织病理改变,免疫荧光观察肾脏组织IgG、C3改变,免疫组化观察Podocin蛋白在肾脏组织的阳性表达情况。结果与正常组相比,其余5组UTP均升高(P <0.05);与模型组相比,各给药组UTP均减少(P<0.05),肾脏组织Podocin蛋白表达增加(P <0.01),TP、ALB有所升高但差异无统计学意义(P> 0.05),Scr、BUN、TC、HDL-C、TAG差异无统计学意义(P> 0.05);与洛丁新组相比,益气活血化瘀汤低、中、高剂量组UTP减少,但差异无统计学意义(P> 0.05),各生化指标差异无统计学意义(P> 0.05),益气活血化瘀汤低、中、高剂量组肾脏组织Podocin蛋白表达增加(P <0.01);益气活血化瘀汤各组之间各指标差异无统计学意义(P>0.05)。结论益气活血化瘀汤可以明显降低膜性肾病大鼠的尿蛋白水平,减少肾脏病理损伤,能够上调肾脏组织Podocin蛋白的表达,延缓膜性肾病的进展。Objective： To observe the effect of Yiqi Huoxue Huayu Decoction on the expression of Podocin protein in renal tissue of rats with membranous nephropathy,and provide a basis for clinical treatment. Methods：SD rats were randomly divided into the normal group（NG）,the model group（MG）,Benazepril group（BG）,Yiqi Huoxue Huayu Decoction groups（LYG,MYG and HYG）. The rats in each group were fed for 4 weeks at corresponding dose,and the urine volume was retained for 24 h per week to detect 24-hour proteinuria（UTP）. The rats were sacrificed at the end of four weeks;the blood were taken out from hearts to detect Scr,BUN,TP,ALB,TC,HDL-C and TAG;the pathological changes of kidney tissues were observed by Masson and PASM staining;IgG and C3 changes in renal tissue were observed by immunofluorescence;the positive expression of Podocin protein in renal tissue was observed by immunohistochemistry. Results： Compared with the NG,UTP increased in the other 5 groups（P〈0.05）;compared with MG,UTP reduced in the drug administration groups（P〈0.05）;the expression of Podocin protein in renal tissue increased（P〈0.01）;TP and ALB increased without statistically significant difference（P〈0.05）,The difference of Scr,BUN,TC,HDL-C and TAG was not statistically significant（P〈0.05）;compared with BG,UTP was induced in LYG,MYG and HYG without statistically significant difference（P〈0.05）,and there was no significant difference in biochemical indexes（P〈0.05）;the expression of Podocin protein in renal tissue of LYG,MYG and HYG increased（P〈0.01）;There was no statistically significant difference between the LYG、MYG and HYG（P〈0.05）. Conclusion： Yiqi Huoxue Huayu Decoction can significantly reduce the urine protein levels of rats with membranous nephropathy,reduce the pathological damage of kidney,up-regulate the expression of Podocin protein in renal tissue,and delay the progress of membranous nephropathy.

关 键 词：益气活血化瘀汤 膜性肾病 PODOCIN 中药治疗 

分 类 号：R285.5[医药卫生—中药学]