一种抗氧化藻蓝蛋白/胶原蛋白复合水凝胶的制备及研究  

Preparation and study of an antioxidant phycocyanin/collagen composite hydrogel

作　　者：王明超 秦松[1] 杜振宁[2] 李文军[1] WANG Mingchao;QIN Song;DU Zhenning;LI Wenjun(Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264005,China;School of Pharmacy,Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong,Key Laboratory of Molecular Pharmacology and Drug Evaluation（Yantai University）,Ministry of Education,Yantai University,Yantai 264005,China)

机构地区：[1]中国科学院烟台海岸带研究所,山东烟台264005 [2]烟台大学新型制剂与生物技术药物研究山东省高校协同创新中心分子药理和药物评价教育部重点实验室(烟台大学),山东烟台264005

出　　处：《功能材料》2018年第11期11102-11106,11113,共6页Journal of Functional Materials

基　　金："十三五"海洋经济创新发展示范项目烟台市创新型海洋医用材料产业孵化聚集创新与示范资助项目(YHCX-SWY-201701);海洋源功能生物材料及医药制剂开发资助项目(KFZD-SW-218)

摘　　要：将罗非鱼鱼皮提取的Ⅰ型胶原蛋白与不同浓度藻蓝蛋白混合,利用胶原蛋白自组装特性制备了复合水凝胶,并考察了复合水凝胶的透气性、微观形貌、红外光谱、溶胀率以及抗氧化性能。结果表明,复合水凝胶透气性显著升高,随着藻蓝蛋白含量增加,水凝胶的微观形貌孔隙和溶胀性增加,红外光谱表明该制备方法未破坏两种蛋白质的特征吸收峰。抗氧化实验中,该复合水凝胶具有DPPH自由基、羟自由基清除力,表现出优异的抗氧化能力。The type I collagen extracted from Tilapia skin was mixed with phycocyanin of different concentrations, and the composite hydrogel was prepared by self-assembly properties of collagen. The permeability, microscopic morphology, infrared spectrum, swelling rate and antioxidant of the composite hydrogel were investigated. Results show that permeability, microscopic pores and swelling rates of composite hydrogel increased significantly with increase of phycocyanin content. Infrared spectra showed that characteristic absorption peaks of these two proteins did not be destroyed by the preparation method. In the antioxidant experiment, composite hydrogel with excellent antioxidant capacity showed scavenging capacity of DPPH free radicals andhydroxyl radical.

关 键 词：藻蓝蛋白 胶原蛋白 复合水凝胶 抗氧化 

分 类 号：R318.08[医药卫生—生物医学工程]