3'-大豆苷元磺酸钠通过金属蛋白酶-9对大鼠脑缺血/再灌注损伤的保护作用  

The protection of 3'daidzein sulfonate sodium with MMP-9 on occludin following cerebral ischemia reperfusion injury in rats

作　　者：刘宗亮[1] 张赛男 黄志华[1] 李良东[1] 黎晓[1] 刘瑞珍[1] 曾靖[1] LIU Zong-liang;ZHANG Sai-nan;HUANG Zhi-hua;LI Liang-dong;LI Xiao;LIU Rui-zhen;ZENG Jing(School of Basic Medicine,Gannan Medical University,Ganzhou,Jiangxi 341000;Postgraduate student,Jiangxi University of Traditional Chinese Medicine,Nanchang,Jiangxi 330006)

机构地区：[1]赣南医学院基础医学院,江西赣州341000 [2]江西中医药大学,在职硕士研究生江西南昌330006

出　　处：《赣南医学院学报》2018年第10期985-987,993,共4页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：国家自然科学基金项目(NO:81160399);江西省高等学校科技落地计划项目(NO:KJLD13085)

摘　　要：目的:研究3'-大豆苷元磺酸钠对大鼠脑缺血/再灌注损伤后金属蛋白酶-9保护作用及其机制。方法:大鼠大脑中动脉栓塞制作局灶性脑缺血/再灌注损伤模型,缺血后10 min,于舌下静脉注射3'-大豆苷元磺酸钠(2. 0 mg·kg^(-1))。再灌注24 h后。取缺血区脑组织制成10%的匀浆,定量PCR检测法测定MMP-9,Gfap,Dlg4,Chrna7 mRNA的表达。结果:与模型组相比,3'-大豆苷元磺酸钠能显著降低MMP-9,GfapmRNA的表达,能显著升高Dlg4和Chrna7 mRNA的表达。结论:3'-大豆苷元磺酸钠对大鼠脑缺血/再灌注损伤的保护作用机制,可能与其降低MMP-9、Gfap mRNA的表达和升高Dlg4、Chrna7 mRNA的表达有关。Objective： To investigate the protective effects of 3′-daidzein sulfonate sodium （DSS） with MMP-9 on occludin following cerebral ischemia reperfusion injury （CIRI） in rats, and the mechanism of these effects. Methods： The rat CIRI model was established by middle cerebral artery occlusion, and received sublingual venous injection of 3′-DSS （2.0 mg·kg^ -1 ） 10 min after ischemia. 24 h after reperfusion, ischemic cerebral tissues were collected and prepared into 10% homogenate, which was used for quantitative PCR assay of the expression of MMP-9,Gfap,Dlg4,Chrna7 mRNA. Results： Compared to the model group, 3′-DSS significantly reduced expression of MMP-9,GfapmRNA, and significantly increased expression of Dlg and Chrna7 mRNA. Conclusion： The protective effects of 3′-DSS on CIRI in rats might be associated with its potential to reduce expression of MMP-9、Gfap mRNA and to increase expression of Dlg4、Chrna7 mRNA.

关 键 词：3′-大豆苷元磺酸钠 脑缺血/再灌注损伤 金属蛋白酶-9 

分 类 号：R285.5[医药卫生—中药学]