唐古特瑞香愈伤组织培养与再生体系建立  

Establishment of the Tissue Culture and Regeneration System of Daphne tangutica

作　　者：季元祖 雷颖[3] 李晓玲[3] 吴利红[3] 赵忠[2] JI Yuan-zu;LEI Ying;LI Xiao-ling;WU Li-hong;ZHAO Zhong(Gansu Provincial Forestry Research Institute,Lanzhou,Gansu 730000,China;Northwest A＆F University,Yangling,Shaanxi 712100,China;Gansu Forestry Professional Technology College,Tianshui,Gansu 741020,China)

机构地区：[1]甘肃省林业科学研究院,甘肃兰州730000 [2]西北农林科技大学,陕西杨陵712100 [3]甘肃林业职业技术学院,甘肃天水741020

出　　处：《西北林学院学报》2018年第6期94-99,共6页Journal of Northwest Forestry University

基　　金：甘肃省重点研发计划项目(2015GS05042)

摘　　要：以唐古特瑞香幼嫩叶片为材料,进行愈伤组织诱导与植株再生试验,以期建立唐古特瑞香再生体系,为其无性系的快速繁殖奠定基础。将幼嫩叶片进行表面灭菌后接种于初代培养基中,初代培养时,存在褐化现象,愈伤诱导前需进行除褐培养,除褐后的外植体经愈伤诱导、不定芽分化、增殖培养、生根培养和幼苗出瓶移栽的试验过程,筛选出了最佳培养方案。除褐培养基以MS+6-BA 1.0mg·L-1+PVP 200mg·L-1效果较好,在此培养基上连续转移3次后褐变消失。愈伤诱导培养基为MS+6-BA 3.0mg·L-1+2,4-D 2.0mgL-1,诱导率95.5%;芽苗分化培养基为MS+ZT 2.0mg·L-1+TDZ 0.1mg·L-1+NAA 0.5mg·L-1,分化率93.2%,平均芽数为8.66条;不定芽增殖培养基为MS+6-BA 2.0mg·L-1+IBA 0.3mg·L-1,增殖倍数为12.21,平均苗高4.81cm;生根培养基为1/2MS+NAA 0.5mg·L-1,生根数平均为3.83条,生根率为88.72%以上;组培苗移栽于珍珠岩中生长良好,成活率达90%以上。本研究初步建立了唐古特瑞香再生体系,为其无性繁殖奠定了理论基础。This study focused on the callus induction and plant regeneration of Daphne tangutica with young leaves for the establishment of an efficient regeneration system.The young leaves were surface sterilized and cultured into primary culture medium.Browning parts resulting from the primary culture were removed before successive inducing callus,and the best culture solution was selected through the process of callus induction,adventitious bud differentiation,proliferation culture,rooting culture and seedling transplanting.The MS medium contained 1.0 mg·L -1 6-BA and 200 mg·L -1 PVP appeared to be the optimum treatment in which browning disappeared from the third transferred medium.The MS medium contained 3.0 mg·L -1 6-BA,2.0 mg·L -1 2,4-D appeared to be the optimum medium for callus induction,from which up to 95.5% induction rate was achieved.The MS medium contained 2.0 mg·L -1 ZT,0.1 mg·L -1 TDZ,0.5 mg·L -1 NAA appeared to be the optimum medium for bud differentiation with up to 93.2% differentiation rate and average 8.66 buds.The MS medium contained 2.0 mg·L -1 6-BA,0.3 mg·L -1 IBA appeared to be the optimum medium for adventitious bud proliferation with 12.21 proliferation rate and average 4.81cm of plantlet height.The 1/2 MS medium contained 0.5 mg·L -1 NAA appeared to be the optimum medium for rooting with over 88.72% rooting rate and the average 3.83 roots.All vitro plantlets grew well after transplanting into the perlite media with over 90% survival rate.The regeneration system established in this study would lay theoretical foundation for asexual reproduction of D.tangutica .

关 键 词：唐古特瑞香 幼嫩叶片 愈伤组织诱导 植株再生 

分 类 号：S722.33[农业科学—林木遗传育种]