肾络宁对膜性肾病小鼠基质金属蛋白酶-9及白介素-10的影响  

Effect of Shenluoning on matrix metalloproteinase-9 and interleukin-10 in mice with membranous nephropathy

作　　者：支勇[1] 王学军[1] 吕艳[1] 曹式丽[1] ZHI Yong;WANG Xue-jun;LV Yan(The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin,300193,China)

机构地区：[1]天津中医药大学第一附属医院

出　　处：《山西中医》2018年第11期47-49,共3页Shanxi Journal of Traditional Chinese Medicine

摘　　要：目的:观察辛通畅络法肾络宁对膜性肾病(MN)模型小鼠基质金属蛋白酶-9 (MMP-9)、白介素-10 (IL-10)的影响。方法:实验Balb/c小鼠采用阳离子化牛血清白蛋白(C-BSA)诱导膜性肾病模型,分为模型组、肾络宁组和MMP-9基因敲除组,另设空白对照组,其中基因敲除组于造模前行MMP-9基因敲除。实验以肾络宁为治疗药物,于实验第0周、第6周末检测各组小鼠尿蛋白,血清IL-10、MMP-9的变化情况。结果:治疗6周后,模型组和肾络宁组、基因敲除组小鼠均出现不同程度的尿蛋白排泄量增加,其中肾络宁组、基因敲除组较模型组小鼠尿蛋白排泄量减少;MMP-9基因敲除组小鼠MMP-9表达显著降低,肾络宁组和模型组有不同程度升高,其中肾络宁组MMP-9表达较模型组降低;模型组、肾络宁组、基因敲除组IL-10表达均匀不同程度降低,肾络宁组、基因敲除组高于模型组(P﹤0.05)。结论:肾络宁对MN模型小鼠具有减少尿蛋白排泄的作用,其机制可能与调节IL-10及MMP-9的表达相关。Objective：To observe the effect of Shenluoning on matrix metalloproteinase-9 （MMP-9）and interleukin-10 （IL-10） in model mice with membranous nephropathy （MN）. Methods：The experimental Balb/c mice were induced as MN model by cationic bovine serum albumin （C-BSA）,and divided into model group,Shenluoning group and MMP-9 gene knock-out group. The blank control group was established in addition. Among them,the gene knock-out group was knocked-out MMP-9 gene before modeling.In the experiment,Shenluoning was taken as therapeutic drug. On the 0th week and 6th weekends of experiment,the changes of urine protein,blood-serum IL-10 andMMP-9 of mice in each group were detected. Results：After 6 weeks of treatment,the mice in model group,Shenluoning group and gene knock-out group increased the excretion of urinary protein in different degrees;among them,the excretion of urinary protein of mice in Shenluoning group and gene knock-out group was lower than that in model group. MMP-9 expression of mice in MMP-9 gene knock-out group was significantly reduced,while MMP-9 expression of mice in model group and Shenluoning group was increased in different degrees;among them,MMP-9 expression of mice in Shenluoning group was lower than that in model group. IL-10 expressions in model group,Shenluoning group and MMP-9 gene knock-out group were lowered in different degrees;Shenluoning group and gene knock-out group were higher than model group （P〈0.05）. Conclusion：Shenluoning has the effect of reducing excretion of urinary protein on MN model mice.Its mechanism may be related to regulating the expressions of IL-10 and MMP-9.

关 键 词：膜性肾病 辛通畅络法 基质金属蛋白酶-9 白介素-10 实验研究 

分 类 号：R285.5[医药卫生—中药学]