法舒地尔对急性乙酰甲胺磷中毒大鼠乙酰胆碱酯酶活性的影响  

The effect of fasudii on the activity of acetylcholinesterase in acute acephate poisoning rats

作　　者：邱冰月 王义霞 田家豪 刘婉诗 施玥 董成祥 燕宪亮 Qiu Bing-yue;Wang Yi-xia;Tian Jia-hao;Liu Wan-shi;Shi Yue;Dong Cheng-xiang;Yan Xian-Hang(Department of Emergency and Rescue Medicine,Xuzhou Medical University,Xuzhou 221002,China)

机构地区：[1]徐州医科大学急救与救援医学系,江苏徐州221002 [2]南京市儿童医院,江苏南京210000 [3]徐州医科大学附属医院急救中心,江苏徐州221002

出　　处：《中国急救医学》2018年第11期996-999,I0002,共5页Chinese Journal of Critical Care Medicine

基　　金：江苏省卫生与计划生育委员会基金面上项目（H201527）;国家级大学生创新创业训练计划项目（201610313013Z）;江苏省大学生创新创业训练计划项目（201610313013）

摘　　要：目的 研究法舒地尔对急性乙酰甲胺磷中毒后乙酰胆碱酯酶（AchE）活性的影响及其可能的作用机制。 方法 SD雄性大鼠共72只，随机分为12组（n=6）：生理盐水对照组（A组）、中毒组（B组）、常规治疗组（按采集标本时间顺序依次为C1、C6、C12、C24、C48组）和法舒地尔联合常规治疗组（按采集标本时间顺序依次为F1、F6、F12、F24、F48组），生理盐水组腹腔注射0.9%氯化钠溶液，其余11组，每只大鼠予以等容积30 mg/kg的乙酰甲胺磷腹腔注射建立中毒模型，C组在中毒后予以肌注阿托品（10 mg/kg）及解磷定（20 mg/kg）治疗，F组予以肌注阿托品（10 mg/kg）、解磷定（20 mg/kg）及腹腔注射法舒地尔（10 mg/kg）治疗，分别于中毒后1、6、12、24及48 h取全血及左室部分组织，采用HE染色方法观察心肌组织的损伤情况，ELISA测定血清AchE水平，免疫印迹测定Bcl-2、Bax进行比较。 结果 C、F组的生存率较B组明显提高，F组生存率较C组明显提高，差异均有统计学意义（P〈0.05）；C、F组血清AchE水平明显高于B组，差异均有统计学意义（P〈0.05）。F组血清AchE水平明显高于C组；C组血清Bax/Bcl-2均在12 h达到峰值，AchE水平在6 h达到峰值，F组血清Bax/Bcl-2在6 h达到峰值，AchE水平在12 h达到峰值。 结论 法舒地尔通过抑制Rho激酶活性一定程度上解除血清中的AchE抑制，并使AchE水平高峰前移，进而降低乙酰胆碱（Ach）蓄积对心肌的损害作用。Objective To study the effect of fasudil on the activity of acetylcholinesterase （AchE） after acute acephate poisoning and its possible mechanism. Methods A total of 72 male SD rats were randomly divided into 12 groups （n = 6） ： normal saline group （group A）, poisoning group （group B）, conventional therapy group （in chronological order： C1, C6, Ci2, C24, C4s group） and conventional therapy combined with fasudil group （in chronological order： Fl, F6, F12, F24, F4s group）. The normal saline group was given 0.9% sodium chloride solution in the abdominal cavity. Each rat of the remaining 11 groups was given 30 mg/kg of acephate in the abdominal cavity to establish acute acephate pesticide poisoning models. The conventional treatment group was intramuscularly injected atropine （10 mg/kg） and resolve pralidoxime （20 mg/kg） after poisoning. The conventional therapy combined with fasudil group was intramuscularly injected atropine （10 mg/kg） and pralidoxime（20 mg/kg） and abdominally injected fasudil （10 mg/kg） for treatment. The complete blood and left ventricular tissue were taken at 1 h, 6 h, 12 h, 24 h and 48 h after poisoning. HE staining was used to observe the myocardial tissue injury. Serum AchE levels were measured by ELISA, and the expression of Bcl -2 and Bax were measured by Western blotting. Compare the changes in the measured indicators for each group. Results The survival rates of group F and group C were significantly higher than that of group B, and the survival rate of group F was significantly higher than that of group C. The difference was statistically significant （ P 〈 0. 05 ）. The levels of serum AchE in group C and group F were significantly higher than those in group B. The difference was statistically significant （ P 〈 0. 05 ）. Compared with group C, the level of serum AchE in group F was significantly higher than group C. In the conventional treatment group, the ratio of Bax/Bcl -2 in serum peaked at 12 h and the levels of acetylcholi

关 键 词：法舒地尔 乙酰胆碱酯酶(AchE) 急性乙酰甲胺磷中毒 急性有机磷农药中毒(AOPP) 

分 类 号：R992[医药卫生—毒理学]