柴芪汤对代谢综合征模型大鼠血清ICAM-1和VCAM-1的影响  

Influence of Chai Qi Decoction on Serum ICAM-1 and VCAM-1 Levels in the Rat Model of Metabolic Syndrome

作　　者：王红梅[1] 葛秉宜 王颖[1] 刘晶[1] 朱梦云[1] 张立平 WANG Hong-mei;GE Bing-yi;WANG Ying;LIU Jing;ZHU Meng-yun;ZHANG Li-ping(Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;Beijing Huairou Hospital of Traditional Chinese Medicine,Beijing 101400,China;Graduate School of Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学东方医院,北京100078 [2]北京市怀柔区中医医院,北京101400 [3]北京中医药大学研究生院,北京100029

出　　处：《中国现代中药》2018年第10期1235-1241,共7页Modern Chinese Medicine

基　　金：国家自然科学基金(81273695);北京中医药大学自主选题青年教师项目(2018-JYBZZ-JS115)

摘　　要：目的:观察柴芪汤对细胞间黏附分子1(ICAM-1)和血管细胞黏附分子1(VCAM-1)的动态影响,探讨柴芪汤防治代谢综合征(Met S)疗效及抗炎作用机制。方法:将8周龄的雄性SD大鼠随机分为正常组、模型组、中药预防组及中药治疗组,其中中药预防组在造模同时灌服柴芪汤,而中药治疗组大鼠由模型组喂养至第8周末时随机抽取并开始给药。于第8周末和第12周末测定总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、空腹血糖(FBG)、空腹胰岛素(FINS),计算胰岛素抵抗指数(IRI)。分别于第4周末、第8周末及第12周末以ELISA法测定ICAM-1、VCAM-1血清浓度。结果:大鼠经高脂高糖高盐饲料喂养8周后,与正常组大鼠相比较,模型组大鼠IRI升高(P <0. 01),血脂TC(P <0. 01)、TG(P <0. 05)、LDL(P <0. 01)升高,出现典型Met S组分,提示造模成功。第12周中药治疗组和预防组与模型组相比,IRI、TC、TG、LDL均较模型组降低(P <0. 01)。中药预防组与治疗组相比,预防组TG较治疗组低(P> 0. 05),预防组IRI、TC值均较低(P <0. 05),预防组LDL也降低(P <0. 01)。各组间HDL均无统计学差异(P> 0. 05)。血清ICAM-1和VCAM-1测定:第12周的中药治疗组低于模型组(P>0. 05);中药预防组低于模型组(P <0. 01);中药预防组低于治疗组(P <0. 05)。结论:通过高脂高糖高盐饮食可成功复制Met S大鼠模型。柴芪汤能调节Met S大鼠的糖脂代谢并下调血清黏附分子,其前期给药的预防作用明显优于后期治疗作用。柴芪汤对于MetS的可能作用机制在于其能降低血清ICAM-1、VCAM-1,改善MetS炎性状态。Objective： To explore the effect of ChaiQi decoction on metabolic syndrome （MetS）and its mechanism. Methods： 8-week old rats were divided into normal group（ NG）, model group（ MG）, preventive Chinese medica- tion group（PCMG） and Chinese medication group（CMG）. NG received a regular diet of rat chow. And other groups received a diet with high fat, sugar and salt. ChaiQi decoction was given to PCMG at beginning of week 1 and to CMG at beginning of week 9. The biochemical markers including serum, total cholesterol （ TC ）, triglyceride （ TG）, low density lipoprotein （ LDL）, high density lipoprotein（HDL） and fasting blood glucose （FBG）were tested using automatic biochemical analyzer. Fasting insulin （FINS） were examined by radioimmunoassay at the end of week 8 and 12, and sICAM-1 and sVCAM-1 were tested by ELISA at the end of week 4, 8 and 12 respectively. Insulin resistance index（IRI） was calculated accordingly. Results： At week 8, the IRI level in MG was significantly higher than that in NG（P 〈0. 01 ） ; the serum TC, TG, LDL levels in MG were significantly higher than those in NG（ P 〈 0. 05 ）. At week 12, the IRI, TC, TG and LDL levels in CMG and PCMG were significantly lower than those in MG（P 〈0. 01 ） ; compared with CMG, the IRI and TC levels and LDL level in PCMG were significantly lower （P 〈0.05 ; P 〈0. 01 ）while there was no significant difference found between CMG and PCMG regarding TG level（P 〉0. 05） and no statistic difference was found among the 4 groups in terms of HDL level. With regard to ICAM-1 and VCAM-1 levels,PCMG was lower than MG（P 〈 0. 01 ）, CMG was lower than MG（P 〉 0. 05 ）, and PCMG was lower than CMG （ P 〈 0. 05 ） at week 12. Conclusion： MetS model can be induced by diet high in fat, sugar, and salt. ChaiQi decoction is effective in inter- vention of MetS in terms of regulating glucose and lipid metabolism and downgrading serum adhesion molecules, while early intervention with the decoction

关 键 词：代谢综合征 柴芪汤 疏肝健脾 炎性因子 胰岛素抵抗 脂代谢紊乱 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]