黄瓜嫩果皮色基因QTL定位分析  

QTL Mapping of Immature Fruit Basal Color in Cucumis sativus L.

作　　者：窦文文 沈镝[1] 麦尔旦.努尔阿伍提 董洪霞[1] 邱杨[1] 李锡香[1] DOU Wen-wen;SHEN Di;NUERAWUTI Maierdan;DONG Hong-xia;QIU Yang;LI Xi-xiang(Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and Germplasm Creation,Ministry of Agriculture,Beijing 100081)

机构地区：[1]中国农业科学院蔬菜花卉研究所/农业部园艺作物生物学与种质创制重点实验室,北京100081

出　　处：《植物遗传资源学报》2018年第6期1138-1142,共5页Journal of Plant Genetic Resources

基　　金：国家科技支撑计划(2013BAD01B04);国家重点研发计划(2016YFD100204)

摘　　要：以黄瓜(Cucumis sativus L.)嫩果皮绿色自交系1613(P1)和嫩果皮白色自交系JD7(P2)为试验材料构建重组自交系(RIL)群体(142个株系),对各株系嫩果皮色进行表型鉴定,利用重测序技术对各自交系进行基因分型,结合表型和基因型数据进行QTL定位研究。结果表明F1黄瓜嫩果皮颜色表现为绿色,并将控制嫩果皮颜色的基因定位到黄瓜第3号染色体上,在3号染色体35511129~39711114区段内定位到成簇分布的3个位点,它们在连锁图谱上的位置分别为1. 01 c M、3. 31 c M和6. 01 c M处,与两翼标记的连锁距离分别为1. 01 c M/0. 09 c M,0. 21 c M/0. 29 c M,0. 11 c M/0. 19 c M。通过生物信息学分析预测出16个候选基因,其中Csa3G904080、Csa3G904100、Csa3G903500和Csa3G902950极有可能是调控黄瓜果实颜色的关键基因。By taking use of 142 recombinant inbred lines （RILs） derived from a cross of green immature fruit inbred line （female parent, 1613 ,P1 ） and white immature fruit inbred line （male parent,JD7, P2）,we performed the quantitative mapping of traits that control the immature fruit skin color. Fl plants showed green peel in immature fruit. By sequencing technology, three QTLs controlling the immature fruit color were allocated to the physical inter- val of 35511129-39711114 on chromosome 3. These loci were located at 1.01 cM,3.31 cM and 6. 01 cM,with the flanking genetic distance of 1.01 cM/0. 09 cM,0. 21 cM/0. 29 cM and 0. 11 cM/0. 19 cM,respectively. By gene annotation, 16 candidate genes were found in the target region, including the genes Csa3G904080, Csa3G904100, Csa3G903500 and Csa3G902950,which are assumed to be the key genes that regulate the color of cucumber fruit.

关 键 词：黄瓜 RIL 嫩果皮色 重测序 QTL定位 

分 类 号：S642.2[农业科学—蔬菜学]