变形链球菌中釉原蛋白序列在龋病预防中的矿化机理初探  

Preliminary study on mineralization mechanism of amelogenin sequences in Streptococcus mutans in caries prevention

作　　者：饶利 张鹏[1] 郭骏 任小华[1] 田鲲[1] RAO Li;ZHANG Peng;GUO Jun;REN Xiao-hua;TIAN Kun Sichuan(People＇s Hospital,Affiliated Hospital of University of Electronic Science and Technology of China,Chengdu,Sichuan 610071,China)

机构地区：[1]电子科技大学附属医院.四川省人民医院,四川成都610071

出　　处：《现代预防医学》2018年第22期4148-4152,4179,共6页Modern Preventive Medicine

基　　金：四川省科技厅应用基础项目;2018JY0057;釉基质蛋白引导釉质样羟基磷灰石非细胞再生的研究;四川省人力资源和社会保障厅留学人员科技活动项目择优资助项目 川人社办发[2016]64生物修饰的高分子模板诱导牙釉质样羟基磷灰石可控生长

摘　　要：目的探讨变形链球菌中的釉原蛋白序列在龋病脱矿、矿化过程中作用及分子机理。方法体外重组、纯化变形链球菌和正常人釉基质中的釉原蛋白功能片段,比较其结构和功能的异同,从蛋白质量、自组装形态、体外成核能力和结晶形态等方面逐一分析区别。结果二者单体形态和大小类似(3～5 nm),都能初级组装为纳米小球,在变形链球菌中纳米球相互重叠形成网状的纳米链,而人类样本中纳米球端端相接组合成规律的纳米链。结论分子水平的结构差异令二者引导形成羟基磷灰石晶体质地和形态大相径庭:在变形链球菌组中杂乱无章、表面粗糙;人釉原蛋白组中规则平行排列、表面致密。此发现对龋病的病因学探讨和预防及釉质再生提出了新的切入点和思路。Objective To explore the role and molecular mechanism of the enamel protein sequence in Streptococcus mutans in the process of caries demineralization and mineralization. Methods Analyzed differences and similarities between human amelogenin and Streptococcus mutans amelogenin from structures and functions. Compared the aspects of protein quality, self-assembly form, in vitro nucleation ability and crystal morphology of them. Results It was found that the morphology and size of the two monomers were similar （3-5nm）, all of which could be assembled into nanospheres. In Streptococcus mutants, nanospheres overlapped with each other to form a network of nanospheres, and the end ends of the nanospheres in human samples were assembled into regular nanoscale chains. Conclusion The structural differences of the molecular level make the two groups lead the formation of hydroxyapatite crystal texture and shape differently： in the group of Streptococcus mutans, the surface is rough; the rules of the human enamel protein group are arranged in parallel and the surface is dense. The discovery provides a new breakthrough point and idea for the etiology and prevention of dental caries and the regeneration of enamel.

关 键 词：釉原蛋白 变形链球菌 生物矿化 龋病 

分 类 号：R181.24[医药卫生—流行病学]