RAD-seq技术在鱼类基因组学中的研究进展  

Research progress of RAD-seq in fish genomics

作　　者：刘涛 李蓉[1] 肖蘅[1] 陈善元[1] LIU Tao, LI Rong, XIAO Heng, CHEN Shan-yuan(School of Life Sciences, Yunnan University, Kunming 650091, China)

机构地区：[1]云南大学生命科学学院,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2018年第6期1283-1289,共7页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金地区科学基金(31560111);云南省"百名海外高层次人才引进计划"项目(W8110305)

摘　　要：限制性酶切位点相关DNA测序(restriction-site associated DNA sequencing,RAD-seq)是在二代测序技术上发展起来的简化基因组测序技术,该技术操作简单、实验成本低、并且简化了基因组的复杂度.鱼类是脊椎动物中物种数最多的类群,由于生活环境的不同,造就了丰富的物种多样性,常作为基因组学的研究对象.目前,在鱼类基因组学研究中,RAD-seq技术广泛应用于鱼类的系统发育、物种分化、遗传图谱、群体遗传和适应性进化等方面的研究.研究主要基于RAD-seq技术在鱼类基因组学中的研究进展进行综述,以期为鱼类资源的保护和合理利用提供参考依据.Restriction-site associated DNA sequencing （RAD-seq） is a reduced-representation sequencing technology developed from next generation sequencing technology.The technology is simple to operate, experimen- tal low cost and greatly simplify the genomic complexity. Fish is the group with the largest number of species in vertebrates and has rich species diversity due to different living conditions, which is often used as research subjects in genomic studies.At present, RAD-seq technology has been widely used in phylogenetic relationships, spe- cies differentiation, genetic map, population genetics and adaptive evolution for fish genomics research.The research progress of RAD-seq in fish genomics is summarized in this paper and reference evidence is thus provided for conservation and rational use of fish resources.

关 键 词：简化基因组测序 RAD-seq 鱼类 

分 类 号：Q953.36[生物学—动物学]