三叶青提取物抗ANIT所致小鼠肝损伤作用的研究  

Study on the trifolium extract in anti ANIT-induced liver injury in mice

作　　者：李萍 吉薇薇[2] 彭昕[2] LI Ping;JI Weiwei;PENG Xin(Department of Pathology,Yinzhou People＇s Hospital in Ningbo City,Ningbo 315000,China;Zhejiang Pharmaceutical College,Ningbo 315100,China)

机构地区：[1]宁波市鄞州人民医院病理科,浙江宁波315000 [2]浙江医药高等专科学校,浙江宁波315100

出　　处：《中国现代医生》2018年第30期32-35,共4页China Modern Doctor

基　　金：浙江省基础公益研究计划项目(LGN18B020001);浙江省公益技术应用研究项目(2017C32075)

摘　　要：目的研究三叶青提取物对α-异硫氰酸萘酯(ANIT)所致肝损伤小鼠的保护作用。方法 2017年1月~2018年2月选择60只小鼠并随机分为6组,分别为对照组、联苯双酯阳性药物组、模型组、三叶青提取物低、中、高剂量组[(1.0、2.0、4.0)g/kg]。采用灌胃ANIT的方式复制急性黄疸型肝炎模型,造模48 h后测其血清中ALT、AST、TBA、TBIL、TNF-α的含量;检测肝组织匀浆中SOD、MDA、GSH-Px的水平。结果三叶青提取物各剂量组均可显著降低TBIL和TNF-α水平(P<0.05),中、高剂量组可以降低血清中ALT、AST、TBA、TBIL、TNF-α含量(P<0.05),各剂量组均显著降低MDA含量,中、高剂量组可显著提高SOD活性(P<0.05),而GSH-Px水平无显著变化。结论三叶青提取物对ANIT所致小鼠肝损伤有一定的保护作用,其保护作用可能与减轻炎症因子产生、促进总胆红素的代谢、降低脂质过氧化程度有关。Objective To study the protective effect of trifolium extract on liver injury induced by α-isothiocyanatoacetate （ANIT） in mice. Methods 60 mice from January 2017 to February 2018 were randomly divided into 6 groups, including control group, bifendate positive drugs group, model group, trifolium extract low, medium and high dose groups[（1.0, 2.0, 4.0） g/kg]. The model of acute jaundice hepatitis was reproduced by intragastric administration of ANIT. Thelevels of ALT, AST, TBA, TBIL, and TNF-α in serum were determined 48 h after modeling. The levels of SOD, MDA,and GSH-Px in liver homogenate were measured. Results The levels of TBIL and TNF-α were significantly decreasedin each dose group（P〈0.05）. The levels of ALT, AST, TBA, TBIL and TNF-α in serum were decreased in mediumand high dose groups（P〈0.05）. Each dose group significantly decreased the MDA content. The medium and high dosegroup could significantly increase SOD activity（P〈0.05）,while GSH-Px levels did not change significantly. ConclusionTrifolium extract has a protective effect on liver injury induced by ANIT in mice. Its protective effect may be related tothe reduction of inflammatory factors, promotion of the total bilirubin metabolism and reduction of the lipid peroxidationdegree.

关 键 词：三叶青 ANIT 肝损伤 抗氧化 

分 类 号：R969.1[医药卫生—药理学]