过敏性紫癜患儿梅毒血清学试验假阳性的探讨  

Serologic false-positive reactions for syphilis in children with allergic purpura

作　　者：石华[1] 李文胜[1] 段义飞[1] 沈川[1] 柳晓琴[1] 甘春玉[1] 江咏梅[1] Shi Hua;Li Wensheng;Duan Yifei;Shen Chuan;Liu Xiaoqing;Gan Chunyu;Jiang Yongmei(Department of Laboratory Medicine,West China Second University Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西第二医院检验科,成都610041

出　　处：《中华临床实验室管理电子杂志》2018年第4期208-211,共4页Chinese Journal of Clinical Laboratory Management(Electronic Edition）

基　　金：四川省科技厅科技支撑项目(2016SZ0053)

摘　　要：目的探讨过敏性紫癜患儿自身抗体对梅毒螺旋体酶联免疫吸附实验(treponema pallidum-enzymelinkedimmunosorbentassay,TP-ELISA)的影响。方法回顾性对比分析2016年1月至2017年10月四川大学华西第二医院38例TP-ELISA假阳性与38例TP-ELISA阴性的过敏性紫癜患儿的临床信息。真阳性组分别对(几组)患儿的血清样本做梅毒血清学ELISA试验、甲苯胺红不加热血清试验(tolulized red unheated serum test,TRUST)、梅毒螺旋体血球凝集试验(treponema pallidum hemagglutination assay,TPHA)、抗核抗体(antinuclear antibody,ANA)试验。并以W检验考察数据是否呈正态分布;用非参数Kurska-Wallis H检验、Mann-Whitney U检验考察组间的相关性。结果假阳性组TP-ELISA的S/Co值显著低于真阳性组(P<0.05),且显著高于真阴性组(P<0.05)。假阳性组患儿中抗核抗体(ANA)检测阳性率达到(42.11%),其中颗粒型占(28.95%),核仁型占(13.16%)。结论过敏性紫癜患儿体内的自身抗体可能会导致TP-ELISA假阳性,预示潜在的自身免疫性疾病。ObjectiveTo investigate the effect of autoantibodies on treponema pallidum-enzyme linked immunosorbent assay （TP-ELISA） in the children with allergic purpura. MethodsA retrospective study was carried out. The clinical information of 38 children with TP-ELISA false positive and 38 children with TP-ELISA negative allergic purpura from January 2016 to October 2017 in West China Second Hospital of Sichuan University was analyzed. The true positive group was tested for syphilis serological ELISA, tolulized red unheated serum test （TRUST）, treponema pallidum hemagglutination assay （TPHA） and antinuclear antibody （ANA） respectively. W test was used to examine whether the data obey normal distribution, non-parametric Kurska-Wallis H test and Mann-Whitney U test were used to analysis the correlation between the two groups. ResultsThe S/Co value of TP-ELISA in false-positive group was signiflcantly lower than that in true positive group （P〈0.05）, and signiflcantly higher than that in true negative group （P〈0.05）. The positive rate of ANA was 42.11% in the false positive group, of which granular type was 28.95% and nucleolar type was 13.16%. ConclusionChildren with allergic purpura who had serologic false-positive reactions for syphilis had a high rate of ANA; it may predict potential autoimmune diseases.

关 键 词：过敏性紫癜 儿童 假阳性 梅毒 

分 类 号：R725.5[医药卫生—儿科] R446.6[医药卫生—临床医学]