氢水干预对反复力竭运动大鼠骨骼肌氧化应激损伤及细胞自噬的影响  

Effects of hydrogen-rich water intervention on rat skeletal muscles′ oxidative damage and autophagy induced by repeated exhaustive exercise

作　　者：徐祖杰 谷红娣[1] 田振军[1] XU Zujie;GU Hongdi;TIAN Zhenjun(School of Physical Education,Shaanxi Normal University,Xi＇an 710119,Shaanxi,China)

机构地区：[1]陕西师范大学体育学院,陕西西安710119

出　　处：《陕西师范大学学报（自然科学版）》2018年第6期116-121,共6页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金(31371199)

摘　　要：为探讨氢水干预对反复力竭运动大鼠骨骼肌氧化应激损伤及细胞自噬的影响,将3月龄雄性SD大鼠30只,体重180~210g,随机分为安静组(C),力竭对照组(EX),力竭氢水组(EH),每组10只。EX和EH组均进行4周反复力竭运动,运动初始速度为15m/min,每5min递增5m/min,至35m/min,运动直至力竭,5d/周,共4周。EH组大鼠于运动前30min给予氢水干预,记录大鼠力竭运动时间。透射电子显微镜观察骨骼肌超微结构变化。检测血清SDH和CK活性,骨骼肌SOD活性、MDA含量和T-AOC水平。Western blotting方法检测骨骼肌mTOR、pmTOR、LC3B-2和P53等自噬相关蛋白表达。结果表明:与EX组比较,氢水干预后,EH组骨骼肌组织超微结构损伤和线粒体肿胀程度均明显减轻,力竭运动时间显著延长(P<0.05)。血清SDH活性显著升高(P<0.05),CK活性显著降低(P<0.05),骨骼肌SOD活性和总抗氧化能力显著升高(P<0.05),MDA含量显著降低(P<0.01),mTOR和p-mTOR蛋白表达显著升高(P<0.05或P<0.01),LC3B-2和P53蛋白表达显著降低(P<0.05)。氢水干预可显著改善反复力竭运动导致的大鼠骨骼肌超微结构损伤、氧化应激损伤及细胞过度自噬现象,提高骨骼肌抗氧化能力和运动耐力。To detect the effects of hydrogen-water intervention on oxidative stress and cell autoph agy in skeletal muscle of rats with repeated exhaustion, 30 male SD rats in the age of 3 months, weighing 1804210 g, were randomly divided into control group （C）, repeated exhaustion group （EX）, and the repeated exhaustion with hydrogen intervention group （EH）, 10 rats in each group. The EX and EH groups were subjected to a four-weeks of repeated-exhaustive exercise. The initial speed of the exercise was 15 m/rain, and increased by 5 m/rain every 5 minutes, the final speed is 35 m/rain until exhaustion, 5 d/week, with a total of 4 week. In EH group, hydro-water was given to rats 30 minutes before exercise. The ultrastructural changes of skeletal muscle were observed by using a transmission electron microscopy. Activity of SDH and CK in serum and SOD activity, MDA content and T-AOC level in skeletal muscle tissue were detected. Western blotting was used to detect the proteins expression of autophagy related proteins in skeletal muscle, mTOR, p-mTOR, LC3B-2 and P53. The results show that compared with the EX group, in the EH group, the ultrastructural damage and mitochondrial swelling were significantly reduced, and the time of exhaustion was significantly prolonged （P〈0.05）. Serum SDH activity increased significantly （P〈0.05）, CK activity decreased significantly （P〈0. 05）, and skeletal muscle tissue SOD activity and total antioxidant capacity significantly increased （P〈0.05）. MDA content decreased significantly （P〈0.01）, mTOR and p-mTOR protein expression was significantly increased（P〈0.05 or P〈0.01）, the LCSB-2 and P53 protein expression was significantly lower （P〈0.05）. Hydrogen water intervention could significantly improve repeatedly exhaustion exercise result in rat skeletal muscle injury, oxidative stress and cell ultrastructure damage excessive autophagy, improving oxidation resistance of the skeletal muscle and exercise endurance.

关 键 词：氢水 力竭运动 骨骼肌 氧化应激 自噬 

分 类 号：G804.4[文化科学—运动人体科学]