降脂理肝汤对高脂饮食诱导的非酒精性脂肪肝大鼠血常规的影响  

Effect of Jiangzhi Ligan decoction on routine blood test results in rats with nonalcoholic fatty liver disease induced by high-fat diet

作　　者：程华初 程婉红 王芳婷 唐聪聪 王一飞 尹抗抗[1] 唐标[1] 徐琦[2] CHENG Hua-chu;CHENG Wan-hong;WANG Fang-ting;TANG Cong-cong;WANG Yi-fei;YIN Kang-kang;TANG Biao;XU Qi(Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;Hunan Academy of Chinese Medicine,Changsha 410006,Hunan,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省中医药研究院,湖南长沙410006

出　　处：《湖南中医杂志》2018年第10期164-167,共4页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省中医药科研计划项目(编号:201664;201844);湖南省卫计委科研计划项目(编号:C2017028);湖南省大学生研究性学习与创新性实验计划项目(湘教办通[2017]116号;序号311)

摘　　要：目的:观察降脂理肝汤对非酒精性脂肪肝(NAFLD)大鼠血常规的影响,探讨降脂理肝汤对NAFLD的作用机制。方法:将SD大鼠随机分为正常组、模型组和降脂理肝汤组,通过高脂饮食诱导NAFLD大鼠模型,分别灌胃给予0. 9%氯化钠注射液、降脂理肝超微汤剂,干预18周后取材,腹主动脉采血,用CA-500血液自动分析仪测定大鼠血常规数据。结果:模型组大鼠的红细胞压积、平均红细胞体积、红细胞体积分布宽度、血红蛋白、平均血红蛋白量、血小板数、白细胞数、单核细胞总数和淋巴细胞总数与正常组相比,差异有统计学意义(P <0. 05或P <0. 01);降脂理肝汤组大鼠红细胞体积分布宽度、血红蛋白、血小板数、白细胞数和单核细胞总数与模型组相比,差异有统计学意义(P <0. 05或P <0. 01),与正常组相比,差异无统计学意义(P> 0. 05);提示已恢复正常水平,说明治疗有效。结论:降脂理肝汤对NAFLD大鼠的红细胞、血小板和白细胞的各项指标均有一定恢复作用,具有改善贫血、减少血小板凝集、降低血液黏稠度和脂质沉积、减少肝脏炎性反应的作用。Objective：To investigate the effect of Jiangzhi Ligan decoction on routine blood test results in rats with nonalcoholic fatty liver disease（ NAFLD） induced by high-fat diet and the mechanism of action of Jiangzhi Ligan decoction on NAFLD. Methods：Sprague-Dawley rats were randomly divided into normal group,model group,and Jiangzhi Ligan decoction group. High-fat diet was given to rats to establish a rat model of NAFLD. The rats were given 0. 9% sodium chloride injection or ultrafine Jiangzhi Ligan decoction by gavage. Related samples were collected after 18 weeks of intervention. Blood samples were collected from the abdominal aorta and the CA-500 automatic blood analyzer was used to obtain routine blood test results. Results：There were significant differences between the model group and the normal group in hematocrit,mean corpuscular volume,red blood cell volume distribution width,hemoglobin,mean corpuscular hemoglobin,platelet count,leukocyte count,total monocyte count,and total lymphocyte count（ P〈0. 05 or P〈0. 01）. There were significant differences between the Jiangzhi Ligan decoction group and the model group in red blood cell volume distribution width,hemoglobin,platelet count,leukocyte count,and total monocyte count（ P〈0. 05 or P〈0. 01）,while there were no significant differences between the Jiangzhi Ligan decoction group and the normal group（ P〉0. 05）,suggesting that these indices had returned to normal and the treatment was effective. Conclusion：Jiangzhi Ligan decoction helps with the recovery of the indices of red blood cell,platelet,and leukocyte in NAFLD rats and can improve anemia,alleviate platelet aggregation,reduce blood viscosity and lipid deposition,and relieve liver inflammation.

关 键 词：NAFLD 大鼠 降脂理肝汤 血常规 实验研究 

分 类 号：R285.5[医药卫生—中药学]