Heterocapsa bohaiensis sp.nov.(Peridiniales:Dinophyceae):a novel marine dinoflagellate from the Liaodong Bay of Bohai Sea,China  

作　　者：XIAO Jie SUN Na ZHANG Yiwen SUN Ping LI Yan PANG Min LI Ruixiang 

机构地区：[1]Key Laboratory of Science and Engineering for Marine Ecology and Environment,the First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China [2]Laboratory for Marine Ecology and Environmental Science,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China [3]Guanghe Crab Industry Limited Company,Panjin 124200,China [4]School of Food and Environment,Dalian University of Technology,Panjin 124221,China

出　　处：《Acta Oceanologica Sinica》2018年第10期18-27,共10页海洋学报（英文版）

基　　金：The NSFC-Shandong Joint Funded Project under contract No.U1406403;the National Natural Science Foundation of China under contract Nos 41506191 and 41306171

摘　　要：A small armed dinoflagellate bloomed in the aquaculture ponds off the coast of Liaodong Bay, Bohai Sea of China, resulting in heavy mortalities of the cultured prawns （Penaeusjaponicus） and larvae of Chinese mitten handed crabs （Eriocheir sinensis）. The bloom-forming species was successfully isolated, and cellular morphology of the specimen was consequently investigated through light, fluorescent and electron microscopy. The small （（14.4±1.6） μm in length） ellipsoid cells show typical Heterocapsa thecal plate arrangement （Po, cp, 5′, 3a, 7″, 6c, 5s, 5′″, 2″″）. The episome is evidently bigger than the hyposome. One to three spherical pyrenoids are located above or beside the large elongated nucleus. The body scale is characterized by a triangle basal plate with one central upright and nine peripheral spines. Above all, Heterocapsa bohaiensis could be distinguished from other Heterocapsa species by the combination of the cell size, morphology, cellular structure and body scale. Sequence analyses of both ITS and LSU regions reveal the significant genetic divergence between H. bohaiensis and other established species in this genus, further supporting novelty of this species. Noticeably, different sample treatment methods resulted in morphological variation of the apical pore complex （APC） of H. bohaiensis, which needs to be taken into account in future study.A small armed dinoflagellate bloomed in the aquaculture ponds off the coast of Liaodong Bay, Bohai Sea of China, resulting in heavy mortalities of the cultured prawns （Penaeusjaponicus） and larvae of Chinese mitten handed crabs （Eriocheir sinensis）. The bloom-forming species was successfully isolated, and cellular morphology of the specimen was consequently investigated through light, fluorescent and electron microscopy. The small （（14.4±1.6） μm in length） ellipsoid cells show typical Heterocapsa thecal plate arrangement （Po, cp, 5′, 3a, 7″, 6c, 5s, 5′″, 2″″）. The episome is evidently bigger than the hyposome. One to three spherical pyrenoids are located above or beside the large elongated nucleus. The body scale is characterized by a triangle basal plate with one central upright and nine peripheral spines. Above all, Heterocapsa bohaiensis could be distinguished from other Heterocapsa species by the combination of the cell size, morphology, cellular structure and body scale. Sequence analyses of both ITS and LSU regions reveal the significant genetic divergence between H. bohaiensis and other established species in this genus, further supporting novelty of this species. Noticeably, different sample treatment methods resulted in morphological variation of the apical pore complex （APC） of H. bohaiensis, which needs to be taken into account in future study.

关 键 词：Heterocapsa harmful algal bloom thecal plate body scale ITS 

分 类 号：Q949.2[生物学—植物学]