牡丹内生真菌MD76的鉴定及次级代谢产物研究  

Identification and Secondary Metabolites of Endophytic Fungus MD76 from Paeonia suffruticosa

作　　者：赵杏利[1] 胡镇杰 侯典云[1] 马占强[1] 郁飞燕[1] ZHAO Xing-li;HU Zhen-jie;HOU Dian-yun;MA Zhan-qiang;YU Fei-yan(College of Agricultural;College of Forestry,Henan University of Science and Technology,Luoyang 471023,China)

机构地区：[1]河南科技大学农学院,河南洛阳471023 [2]河南科技大学林学院,河南洛阳471023

出　　处：《中国药学杂志》2018年第21期1826-1830,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金项目资助(U1404829);河南省高等学校重点科研项目资助(18A180013;18A210009);河南省自然科学基金(182300410075)

摘　　要：目的对一株具有抗菌活性的牡丹内生真菌MD76进行鉴定并对其次级代谢产物进行分离。方法采用形态学观察结合5. 8s r DNA ITS序列分析鉴定菌株MD76的种类;利用硅胶、凝胶柱层析等技术对该菌的大米发酵产物乙酸乙酯浸提物进行分离,并通过波谱数据对分离化合物鉴定。结果鉴定菌株MD76为首都叶点霉Phyllosticta capitalensis,并从该菌发酵物中分离到7种化合物:苯甲酸(1)、对羟基苯甲酸(2)、对羟基苯丙酸(3)、芍药苷(4)、氧化芍药苷(5)、没食子酸(6)、芹菜素(7)。结论首都叶点霉P. capitalensis为首次从牡丹健康组织中分离获得,7种化合物均为首次从该种真菌中提取鉴定。In order to identify the endophytic fungus MD76 and isolate its secondary metabolite. METHODS The identification of strain MD76 is based on morphological characteristics and sequence alignment analysis of 18s rDNA ITS, the secondary metabolites were isolated by chromatography technology ; and their structures were elucidated by extensive spectroscopic analysis. RESULTS The strain MD76 was identified as Phyllosticta capitalensis ; and seven compounds were gained, including benzoic acid （ 1 ）, 4 - hydroxybenzoic acid（ 2 ）, 4 - hydroxybenzene propanoic acid （ 3 ）, paeoniflorin （ 4 ）, oxypaeoniflorin （ 5 ）, gallic acid （ 6 ）, apigenin （ 7 ）. CONCLUSION The endophytic fungus P. capitalensis was gained firstly from Paeonia suffruticosa and seven compounds were isola- ted from P. capitalensis for the first time.

关 键 词：牡丹 首都叶点霉 内生真菌 次级代谢产物 单萜苷 

分 类 号：R965[医药卫生—药理学]