猪伪狂犬病病毒HB-11株的分离鉴定及gC基因的分析  

Isolation and identification of porcine pseudorabies virus(PRV) strain HB-11 and analysis of gC gene

作　　者：马晶晶 吴碧清 高俊锋 郑良益 舒银辉 赖志 MA Jing-jing;WU Bi-qing;GAO Jun-feng;ZHENG Liang-yi;SHU Yin-hui;LAI Zhi(Shanghai Chuanghong Biotech Co.,Ltd,Shanghai 201619,China;Wuhan ChoppoBiological Corporation Co.,Ltd,Wuhan 430070,China)

机构地区：[1]上海创宏生物科技有限公司,上海松江201619 [2]武汉中博生物股份有限公司,湖北武汉430070

出　　处：《中国兽医杂志》2018年第8期28-32,共5页Chinese Journal of Veterinary Medicine

基　　金：上海市中小型企业科学技术创新扶持资金项目(1202H115600)

摘　　要：将某猪场发病死亡猪的脑和肺组织接种BHK-21细胞,连传5代均出现典型细胞病变,表明分离到1株病毒,病毒含量为108. 33TCID50/mL。该毒株能被猪伪狂犬病病毒标准阳性血清中和,接种家兔、小鼠和仔猪均出现典型伪狂犬病症状,表明分离株为猪伪狂犬病病毒,命名为PRV HB-11株。gC基因的遗传进化分析显示,HB-11株与近年来流行的变异株同源性为99. 3%~99. 4%,与Bartha株的同源性为94. 8%,其gC基因序列相对于Bartha株序列有7个连续氨基酸的插入(158AAASTPA164),该突变插入的生物学意义有待于进一步研究。One virus was isolated from the brain and lung organs of diseased piglets from a pig farm. The virus induced typicalcytopathic effect（CPE） after being inoculated in BHK-21cell. The virus TCID50 was 108. 33TCID50 / ml. The virus was neutralized bystandard positive serum of pseudorabies virus. and cause typical pseudorabies clinical symptom and death infected rabbits、mice andpiglets, which indicated the isolated virus was pseudorabies virus（PRV） and it was named virus HB-11 strain. Phylogenetic tree ofgC genes showed that the gC gene of HB-11 shared 99. 3% to 99. 4% identity with variant stains prevailing in China in recent yearsand 94. 8% identity with the classic strain Bartha . The HB-11isolates had a unique^158AAASTPA^164insertion in amino acid sequenceof gC, which were considered to be molecular marker of the variants PRV.

关 键 词：猪伪狂犬病病毒 分离 鉴定 GC基因 

分 类 号：S852.651[农业科学—基础兽医学]