萱花草总皂苷的微波提取及其抗肿瘤活性研究  

Study on Microwave Extracting and Antitumor Activity of Total Saponins from H.emerocallisfulva

作　　者：刘立新[1] 张雪松[2] 张羽男[1] 叶之慧[3] 孙晶[2] 于海涛[2] 李晶[4] 王晶[2] 罗文泽 张强[5] LIU Li-xin;ZHANG Xue-song;ZHANG Yu-nan;YE Zhi-hu;SUN Jing;YU Hai-tao;LI Jing;WANG Jing;LUO Wen-ze;ZHANG Qiang(School of Pharmacological Sciences;First Affiliated Hospital;Department of Stomatology,First Affiliated Hospital;School of Basic Medical Sciences;School of Public Health,Jiamusi University,Jiamusi 154007,Heilongjiang,China)

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007 [2]佳木斯大学附属第一医院,黑龙江佳木斯154007 [3]佳木斯大学附属口腔医院,黑龙江佳木斯154007 [4]佳木斯大学基础医学院,黑龙江佳木斯154007 [5]佳木斯大学公共卫生学院,黑龙江佳木斯154007

出　　处：《湖北农业科学》2018年第21期93-96,共4页Hubei Agricultural Sciences

基　　金：黑龙江省卫生计生委科研课题(2016-297;2018-492);国家自然科学基金项目(81641120)

摘　　要：以微波功率、提取时间和原料固液比为主要因素,采用L9(33)正交试验进行微波辅助提取萱花草(H.emerocallisfulva)总皂苷工艺条件的优化,所得最佳提取工艺条件为微波功率420 W、提取时间45 s、原料固液比1∶50(g∶mL)。采用SRB法研究萱花草总皂苷对人肝癌HepG2细胞的体外抗肿瘤活性,试验结果表明萱花草总皂苷对人肝癌HepG2细胞株有良好的抗肿瘤活性,且优于人参皂苷Rb1的阳性对照组。Using microwave pouter, extracted time and solid-liquid ratio as main factors, the L9（3^3） orthogonal design was used for optimizing the extraction conditions of total saponins from H.emerocallisfulva. The optimum conditions were as：microwave power at 420 W,45 s of extracted time and solid-liquid ratio at l：50（g：mL）. Antitumor activity of total saponins from H.emerocallisfulva was studied by the SRB assay,the result shows that total saponins from H.emerocallisfulva demonstrated good antitumor activity on human hepatoma HepG2 cells,and better than the ginsenosides Rb1.

关 键 词：萱花草(H.emerocallisfulva) 总皂苷 微波辅助提取 抗肿瘤活性 

分 类 号：R284.2[医药卫生—中药学]