Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules  

作　　者：Florin-Dan Popescu Mariana Vieru 

机构地区：[1]Department of Allergology, "Carol Davila" University of Medicine and Pharmacy [2]Department of Allergology and Clinical Immunology, "Nicolae Malaxa" Clinical Hospital

出　　处：《World Journal of Methodology》2018年第3期17-36,共20页世界方法学杂志

摘　　要：Molecular-based allergy diagnosis for the in vitro assessment of a patient immunoglobulin E(IgE) sensitization profile at the molecular level uses allergen molecules(also referred to as allergen components), which may be well-defined, highly purified, natural allergen components or recombinant allergens. Modern immunoassay methods used for the detection of specific Ig E against aeroallergen components are either singleplex(such as the fluorescence enzyme immunoassay with capsulated cellulose polymer solid-phase coupled allergens, the enzyme-enhanced chemiluminescence immunoassay and the reversed enzyme allergosorbent test, with liquid-phase allergens), multiparameter(such as the line blot immunoassay for defined partial allergen diagnostics with allergen components coating membrane strips) or multiplex(such as the microarraybased immunoassay on immuno solid-phase allergen chip, and the two new multiplex nanotechnology-based immunoassays: the patient-friendly allergen nanobead array, and the macroarray nanotechnology-based immunoassay used as a molecular allergy explorer). The precision medicine diagnostic work-up may be organized as an integrated "U-shape" approach, with a "top-down" approach(from symptoms to molecules) and a "bottomup" approach(from molecules to clinical implications), as needed in selected patients. The comprehensive and accurate Ig E sensitization molecular profiling, with identification of the relevant allergens, is indicated within the framework of a detailed patient's clinical history to distinguish genuine Ig E sensitization from sensitization due to cross-reactivity(especially in polysensitized patients), to assess unclear symptoms and unsatisfactory response to treatment, to reveal unexpected sensitizations, and to improve assessment of severity and risk aspects in some patients. Practical approaches, such as anamnesis molecular thinking, laboratory molecular thinking and postmolecular anamnesis, are sometimes applied. The component-resolved diagnosis of the specific IgE repertoire has aMolecular-based allergy diagnosis for the in vitro assessment of a patient immunoglobulin E （IgE） sen-sitization profile at the molecular level uses allergen molecules （also referred to as allergen components）, which may be well-defined, highly purified, natural allergen components or recombinant allergens. Modern immunoassay methods used for the detection of specific IgE against aeroallergen components are either singleplex （such as the fluorescence enzyme immunoassay with capsulated cellulose polymer solid-phase coupled allergens, the enzyme-enhanced chemiluminescence immunoassay and the reversed enzyme allergosorbent test, with liquid-phase allergens）, multiparameter （such as the line blot immunoassay for defined partial allergen diagnostics with allergen components coating membrane strips） or multiplex （such as the microarray-based immunoassay on immuno solid-phase allergen chip, and the two new multiplex nanotechnology-based immunoassays： the patient-friendly allergen nano-bead array, and the macroarray nanotechnology-based immunoassay used as a molecular allergy explorer）. The precision medicine diagnostic work-up may be organized as an integrated “U-shape” approach, with a “top-down” approach （from symptoms to molecules） and a “bottom-up” approach （from molecules to clinical implications）, as needed in selected patients. The comprehensive and accurate IgE sensitization molecular profiling, with identification of the relevant allergens, is indicated within the framework of a detailed patient’s clinical history to distinguish genuine IgE sensitization from sensitization due to cross-reactivity （especially in polysensitized patients）, to assess unclear symptoms and unsatisfactory response to treatment, to reveal unexpected sensi-tizations, and to improve assessment of severity and risk aspects in some patients. Practical approaches, such as anamnesis molecular thinking, laboratory molecular thinking and postmolecular anamnesis, are sometimes applied. The compone

关 键 词：Singleplex Multiplex IMMUNOGLOBULIN E SENSITIZATION AEROALLERGENS Immunoassays 

分 类 号：O1[理学—数学]