阴炎净洗液对大鼠接触性皮炎湿疹的抗炎作用及其分子机制研究  

Study of anti-inflammatory effect and molecular mechanism of Yinyanjing Lotion

作　　者：谭谦[1] 欧阳波[1] 肖作奇 TAN Qian;OUYANG Bo;XIAO Zuoqi(Department of Pharmaceutical Preparation,Hu′nan Provincial Maternal and Child Health Care Hospital,Hu′nan Province,Changsha,410008,China)

机构地区：[1]湖南省妇幼保健院药学制剂部,湖南长沙410008

出　　处：《中国医药导报》2018年第33期13-16,共4页China Medical Herald

基　　金：湖南省中医药科研计划项目(201671)

摘　　要：目的探究阴炎净洗液对大鼠接触性皮炎湿疹(ACD)的抗炎作用及其分子机制。方法将50只雌雄各半的SPF级SD大鼠按随机数字表法分为五组,分别为空白对照组(纯化水50μL)、ACD模型组(纯化水50μL)、阳性对照组(地塞米松磷酸钠注射液50μL)、低剂量治疗组(阴炎净洗液50μL)和高剂量治疗组(2倍浓缩阴炎净洗液50μL),每组10只,每天相应药物涂抹右耳2次,给药10 d。观察皮肤变应性反应和肿胀度,利用免疫组织化学法和蛋白免疫印迹实验检测磷酸化细胞外调节蛋白激酶(pERK1/2)和核因子κB(NF-κB)的表达水平。结果与空白对照组比较,ACD模型组大鼠耳肿胀度明显升高(P <0.01)。与ACD模型组比较,高剂量治疗组和低剂量治疗组的耳肿胀度均明显降低(P <0.01)。与空白对照组比较,ACD模型组大鼠耳组织中p ERK1/2和NF-κB的蛋白表达明显升高(P <0.01)。与ACD模型组比较,高剂量治疗组和低剂量治疗组的pERK1/2和NF-κB的蛋白表达均明显降低(P <0.05或P <0.01)。结论阴炎净洗液对ACD大鼠有抗炎作用,其机制可能是依赖于对pERK_(1/2)/NF-κB信号通路的抑制作用。Objective To investigate anti-inflammatory effect and molecular mechanism of Yinyanjing Lotion on allergic contact dermatitis （ACD） rats. Methods Fifty half male and half female SPF grade SD rats were randomly divided into 5 groups according to random number table method, including blank control group （purified water 50 μL）, ACD model group （purified water 50 μL）, positive control group （Dexamethasone Sodium Phosphate Injection 50 μL）, low dose treatment group （Yinyanjing Lotion 50 μL） and high dose treatment group （2 times concentrated Yinyanjing Lotion 50 μL）, with 10 rats in each group. The right ears were smeared with drugs twice a day for 10 days. The allergic reaction and swelling degree of the skin were observed, and the expression levels of phosphorylated extracellular regulated protein kinases （pERK1/2） and nuclear factor κB （NF-κB） were detected by immunohistochemistry and Western blot. Results Compared with the blank control group, the ear swelling degree of rats in the ACD model group was significantly increased （P 〈 0.01）. Compared with the ACD model group, the ear swelling degree of rats in the high dose treatment group and low dose treatment group was significantly decreased （P 〈 0.01）. Compared with the blank control group, the protein expression of pERK1/2 and NF-κB in the ACD model group was significantly up-regulated （P 〈 0.01）. Compared with the ACD model group, the protein expression of pERK1/2 and NF-κB in the high dose treatment group and low dose treatment group was significantly down-regulated （P 〈 0.05 or P 〈 0.01）. Conclusion Yinyanjing Lotion has an anti-inflammatory effect on ACD rats, and the mechanism may be achieved by inhibition of pERK1/2/NF-κB signaling pathway.

关 键 词：阴炎净 接触性皮炎 核因子&kappa B通路 PERK1/2 

分 类 号：R758.22[医药卫生—皮肤病学与性病学]