不同产地北沙参多糖的性质和生物活性研究  

Studies on the Physicochemical Properties and Biological Activities of Glehnia littoralis Polysaccharides from Different Locations

作　　者：景永帅[1] 张丹参[1] 苏蕾 张瑞娟 吴兰芳 郑玉光 JING Yong-shuai;ZHANG Dan-shen;SU Lei;ZHANG Rui-juan;WU Lan-fang;ZHENG Yu-guang(College of Chemistry and Pharmaceutical Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,Hebei,China;College of Pharmacology,Hebei University of Chinese Medicine,Shijiazhuang 050200,Hebei,China)

机构地区：[1]河北科技大学化学与制药工程学院,河北石家庄050018 [2]河北中医学院药学院,河北石家庄050200

出　　处：《食品研究与开发》2018年第24期21-26,共6页Food Research and Development

基　　金：第四次全国中药资源普查项目(Z135080000022);河北省自然科学基金项目(H2016208059;H2016208058;H2017423023);河北省高等学校科学技术研究项目(ZD2016009);河北省现代农业技术体系中药材产业创新团队建设(20170401458-2)

摘　　要：对10个产地北沙参中多糖的提取率、可溶性总糖含量、黏度、分子量分布、抗氧化及降血糖活性进行了研究。结果表明:产地不同,北沙参多糖的理化性质和生物活性存在差异,但具有相似的紫外、红外光谱学性质及分子量分布。多糖提取率范围在(13.20±0.07)%～(26.75±0.01)%之间,可溶性总糖含量范围为(81.28±0.02)%～(89.10±0.10)%,黏度范围为(6.55±0.05)Pa·s～(9.40±0.10)Pa·s;其中产地为河北安国的北沙参多糖对DPPH和羟基自由基的清除率以及对α-葡萄糖苷酶的抑制率最高,半数清除率浓度(half maximal inhibitory concentration,IC50)分别为0.237、0.564、10.614 mg/mL。The extraction rate, soluble total sugar content, viscosity, molecular weight distribution, antioxidant and hypoglycemic activities of Glehnia/ittoralis polysaccharides from 10 habitats were studied. The results showed that polysaccharides from different origins had diiterent physicochemical properties and biological activities, but they had similar ultraviolet and infrared spectroscopy properties and molecular weight distribution. The extraction rate of polysaccharides ranged from （13.20±0.07） % to （26.75 ±0.01 ） %, the total soluble sugar content ranged from （81.28±0.02） % to （89.10±0.10） %, and the viscosity ranged from （6.55±0.05 ） Pa·s to （9.40＋0.10） Pa＂ s. The polysaccharides from the G/ehnia/ittoralis of Hebei Anguo had the highest scavenging rate of DPPH and hydroxyl radicals and the highest inhibition rate of α-glucosidase, and the half maximal inhibitory concentration （IC50） was 0.237, 0.564 mg/mL and 10.614 mg/mL, respectively.

关 键 词：北沙参 多糖 理化性质 抗氧化 Α-葡萄糖苷酶抑制剂 

分 类 号：R284.2[医药卫生—中药学]