藏红花侧芽中L-瓜氨酸的提取与测定  

Extraction and determination of L-citrulline in the lateral bud of saffron

作　　者：李数数 王静 侯静 彭彤[1] 何韵云 魏琴[2] 白洁[1] LI Shu-Shu;WANG Jing;HOU Jing;PENG Tong;HEYun-Yun;WEI Qin;BAI Jie(Key Laboratory Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University,Chengdu 610065,China;Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education,Yibin University,Sichuan 644000,China)

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610065 [2]宜宾学院香料植物资源开发与利用四川省高校重点实验室,宜宾644000

出　　处：《四川大学学报（自然科学版）》2018年第6期1297-1300,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省高校重点实验室开放基金(2016XLZ002);西红花规模化生产技术服务(HS20100601106684)

摘　　要：对藏红花(Crocus sativus L.)侧芽中的L-瓜氨酸进行定性定量分析,并对提取方法进行优化.样品采用超声波法提取,2,4-二硝基氟苯(FDNB)柱前衍生,通过超高效液相色谱进行分析.实验结果表明L-瓜氨酸在0.98～62.50μg/mL(R2=0.9998)范围内线性关系良好,检测限(S/N=3)0.2440ng/mL,样品平均回收率为101.15%(n=6,RSD=1.00%).藏红花侧芽中含有丰富的L-瓜氨酸,提取条件经优化后,测得藏红花侧芽中L-瓜氨酸的含量约为26.69mg/g.L-citrulline in the lateral bud of saffron was quantitatively analyzed for the first time and the extraction method was optimized. After ultrasonic extraction, the samples were derived with 2,4-dinitrofluorobenzene （FDNB） and analyzed by Ultra High Performance Liquid Chromatography. The results showed that a good linear relationship was obtained for L-citrulline in the concentration range of 0.98~62.50 μg/mL with R ^2 = 0.9998,the detection limit of L- citrulline was 0.2440ng/mL（S/N=3）, and the average recovery was 101.15% （ n =6, RSD =1.00% ）. After the extraction method was optimized,the content of L-citrulline in the lateral bud of saffron was determined to be about 26.69 mg/g.

关 键 词：藏红花侧芽 L-瓜氨酸 超高效液相色谱法 提取 含量测定 

分 类 号：Q945[生物学—植物学]