一株来自青藏高原土壤日本曲霉菌的分离鉴定及其杀东亚飞蝗活性研究  

Investigation on the insecticidal potential of Aspergillus japonicas D3-19 isolated from the Tibetan plateau against Locusta migratoria manilensis(Meyen)(Orthoptera:Acrididae)

作　　者：查艳梅 杨娜 石佳杏 陶科[1] 金洪[1] 侯太平[1] ZHA Yan-Mei;YANG Na;SHI Jia-Xing;TAO Ke;JIN Hong;HOU Tai-Ping(Key Laboratory of Bio-resource and Eco-environment,Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610065

出　　处：《四川大学学报（自然科学版）》2018年第6期1307-1312,共6页Journal of Sichuan University(Natural Science Edition)

基　　金："十三五"国家重点研发计划(2016YFC0502004)

摘　　要：采用稀释涂布法从青藏高原采集的土样中分离得到一株具有杀蝗虫活性真菌D3-19.通过形态学观察以及ITS序列的分析,初步将D3-19鉴定为曲霉属日本曲霉(Aspergillus japonicas).室内活性测定结果表明日本曲霉D3-19发酵液和孢子均有杀东亚飞蝗活性.其中,真菌发酵液对东亚飞蝗校正死亡率为:96h(86.67%),144h(100%).与此同时,真菌孢子悬液对东亚飞蝗的LC50(致死中浓度)为3.42×105孢子/mL.研究结果表明,3×108孢子/mL的D3-19孢子悬液对不同年龄阶段东亚飞蝗均有致死效果.其LT50(半致死时间)值分别为:羽化成虫(155h),四至五龄成虫(104.8h),三龄幼虫(54.6h),二龄幼虫(30.6h).By using dilution coating method a fungus D3-19 with insecticidal activity against locusts was isolated from soil of the Qinghai-Tibet Plateau. By the morphological observation and ITS sequence analysis, D3-19 was preliminary identification as A.japonicas . Indoor bioassay results showed that both fermentation liquor and conidial suspension of A.japonicas D3-19 had insecticidal activity against L. migratoria . For 96 and 144 h of treatment, The corrected mortalities of the A. japonicas D3-19 fermentation liquor against L.migratoria were 86.67% and 100%, respectively. Meanwhile, the LC 50 value of the A. japonicas D3-19 conidia suspension against L.migratoria was 3.42×10^5 conidia/mL. The result showed that, 3×10^8 conidia/mL conidial suspension of A. japonicas D3-19 had insecticidal activity against all life stages of L. migratoria , and the A. japonicas D3-19 conidia suspension against emerging adults, fourth to fifth instars, third instars larvae, and second instars larvae were 155 h, 104.8 h, 54.6 h, and 30.6 h, respectively.

关 键 词：青藏高原 东亚飞蝗 日本曲霉 杀虫活性 

分 类 号：S43[农业科学—农业昆虫与害虫防治]