3种不同梅毒血清学实验方法在临床中的应用价值及效能比较  

Comparation of clinical values of 3 different methods of syphilis serology in clinical practice

作　　者：符颖 陈菊香 何瑞英 王声兴[2] FU Ying;CHEN Juxiang;HE Ruiying;FANG Shengxing(Wenchang Dermatology and Venereal Disease Control Center,Wenchang 571300,Hainan,China;Department of Urology,The First Hospital Affiliated to Hainan Medical University,Haikou 570102,Hainan,China)

机构地区：[1]文昌市皮肤性病防治中心,海南文昌571300 [2]海南医学院第一附属医院泌尿外科,海口570102

出　　处：《中国性科学》2018年第10期121-123,共3页Chinese Journal of Human Sexuality

基　　金：海南省自然科学基金资助项目(20168304)

摘　　要：目的:比较3种不同梅毒血清学实验方法在临床中的应用价值。方法:对文昌市皮肤性病防治中心2013年4月至2017年4月收治的548例患者的临床资料进行统计分析,依据感染梅毒情况将这些患者分为感染组(n=354)和未感染组(n=194)两组,运用ELISA法、TPPA法、TRUST法检测所有患者血清标本中的梅毒螺旋体抗体,然后对两组患者3种不同梅毒血清学实验方法检测梅毒抗体阳性情况、3种不同梅毒血清学实验方法的检测效能、应用价值进行统计分析。结果:3种不同梅毒血清学实验方法检测感染组患者的梅毒抗体阳性率均显著高于未感染组,差异具有统计学意义(P <0. 05); ELISA法、TPPA法检测感染组患者的梅毒抗体阳性率均显著高于TRUST法,差异具有统计学意义(P <0. 05); ELISA法、TPPA法检测的敏感性、特异性、阳性预测值、阴性预测值均显著高于TRUST法,差异具有统计学意义(P <0. 05); 3种不同梅毒血清学实验方法同时显示梅毒抗体阳性310例,阴性2例; ELISA法、TPPA法显示阳性而TRUST法显示阴性34例。结论:ELISA法、TPPA法较TRUST法在临床中具有较高的应用价值,能够将漏诊率及误诊率降低到最低限度,值得推广。Objective： To compare the clinical values of 3 different serological methods of syphilis in clinical practice. Methods： The clinical data of 548 cases of patients in our center from April 2013 to April 2017 were statistically analyzed,these patients were divided into infection group（ n = 354） and non infection group（ n = 194）two groups according to the situations of syphilis infection,the serum specimens of Treponema pallidum antibody of all patients were detected by ELISA method,TPPA method,TRUST,then the detection syphilis antibody positive cases of 3 different serological tests of the two groups,the efficacy and application values of 3 different methods of serological tests were statistically analyzed. Results： The positive rate of Syphilis Serological Detection of 3 different experimental methods of the infection group were significantly higher than the non infection group（ P〈0. 05）; the detection positive rates of syphilis antibody of ELISA method,TPPA method were significantly higher than TRUST method（ P〈0. 05）; The the detection sensitivity,specificity,positive predictive values and negative predictive values of ELISA method,TPPA method were significantly higher than TRUST method（ P〈0. 05）; 3 different methods of serological tests for syphilis all showed positive syphilis antibody 310 cases,negative 2 cases; ELISA method,TPPA method showed positive and TRUST method showed negative 34 cases. Conclusions： ELISA and TPPA are more valuable than TRUST in clinical practice,can reduce the misdiagnosis rate to the minimum,so is worthy of popularizing.

关 键 词：甲苯胺红不加热血清试验 梅毒螺旋体明胶凝集试验 梅毒酶联免疫吸附试验 应用价值 

分 类 号：R759.1[医药卫生—皮肤病学与性病学] R446.6[医药卫生—临床医学]