HPLC测定天麻配方颗粒中天麻素和对羟基苯甲醇的含量  

HPLC Method for Determination of Gastrodin and 4-Hydroxybenzyl Alcohol in Tianma Formula Granule

作　　者：相继芬 邓辰辰 刘圆[1,2] 韩蕾 徐桂红[1,2] 萧伟[1,2] XIANG Ji-fen;DENG Chen-chen;LIU Yuan;HAN lei;XU Gui-hong;XIAO Wei(Jiangsu Kanion Parmaceutical Co.,Ltd.Lianyungang 222001,Jiangsu,China;The Key Laboratory jbr the New Technique Research of TCM Extraction and Purification,Lianyungang 222001,Jiangsu,China)

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药提取精制新技术重点研究室,江苏连云港222001

出　　处：《中国现代中药》2018年第11期1440-1443,共4页Modern Chinese Medicine

摘　　要：目的:建立天麻配方颗粒中天麻素和对羟基苯甲醇含量测定方法,为其质量控制提供可靠依据。方法:采用高效液相色谱法,Welch Ultimate LP-C18色谱柱(250 mm×4. 6 mm,5μm),以乙腈-0. 1%磷酸(4∶96)为流动相,检测波长为220 nm,流速为0. 8 m L·min-1,柱温为30℃。结果:天麻素在49. 31～986. 2μg·m L-1范围内呈良好线性(r=0. 999 6),加样回收率平均值为102. 68%,RSD值为1. 66%;对羟基苯甲醇在7. 589～151. 78μg·m L-1范围内呈良好线性(r=0. 999 6),加样回收率平均值为97. 88%,RSD值为1. 96%。结论:该方法精密度、重复性和分离效果好,分析快速、准确,适用于对天麻中天麻素和对羟基苯甲醇的定性、定量研究。Objective： To establish an HPLC method for the determination of gastrodin and hydroxyl benzyl alcohol in Tianma Formula Granule. Method： HPLP was performed on Welch Ultimate LP-C18 eolunm（250 mnl x 4.6 mnl, 5 txm）. The mobile phase consisted of aeetonitrile and 0. 1% phosphate（4：96）. The detection wavelength was at 220 nm, the flow rate was 0. 8 mL-1 min-1 , The eolunm temperature was at 30 ℃. Results： Gastrodin showed a good linearity（ r = 0. 999 6）in the range of 49. 31 -986. 2 μg .mL-1, the average recovery rate was 101.42% and RSD was 0. 42%. 4-hydroxy benzyl alcohol in the range of 7. 589 -151.78 μg .mL-1 range, showed a good linearity（ r = 0. 999 6）, the average recovery rate was 101.42% and RSD was 1.31%. Conclusion： This method is quick, precise, rapid and accurate, and suitable for the qualitative and quantitative research on gastrodin and 4-hydroxybenzyl alcohol in Tianma Formula Granule.

关 键 词：天麻配方颗粒 天麻素 对羟基苯甲醇 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] R286.0[理学—化学]