Next generation sequencing in oral disease diagnostics  

作　　者：Gokul Sapna Sridharan Gokul 

机构地区：[1]Department of periodontics, Nair Hospital Dental College [2]Oral Pathology and Microbiology, YMT Dental College and Hospital

出　　处：《World Journal of Stomatology》2018年第2期6-10,共5页世界口腔医学杂志

摘　　要：DNA sequencing is the method of identifying the precise order of DNA nucleotides within a molecule. The information of DNA sequencing is of prime requisite for basic biological research as well as in various clinical specialties.They can be used to determine the individual genetic sequence, larger genetic regions, chromosomes as well as to sequence RNA and proteins. Since the first DNA sequencing in 1970s, there has been tremendous advancements in the technologies aimed to determine the entire human genome. The need for rapid and accurate sequencing of human genome has resulted in the introduction of next generation sequencing(NGS) technology. NGS refers to the secondgeneration DNA sequencing technologies where millions of DNA can be sequenced simultaneously. Some of the next gen sequencing methods employed are Roche/454 life science, Illumina/Solexa, SOLiD system and HeliScope.Application of NGS in decoding the genomic database of various oral diseases may possess therapeutic and prognostic value. This presentation provides an overview of the basics of NGS and their potential applications in oral disease diagnostics.DNA sequencing is the method of identifying the precise order of DNAnucleotides within a molecule. The information of DNA sequencing is of primerequisite for basic biological research as well as in various clinical specialties.They can be used to determine the individual genetic sequence, larger geneticregions, chromosomes as well as to sequence RNA and proteins. Since the firstDNA sequencing in 1970s, there has been tremendous advancements in thetechnologies aimed to determine the entire human genome. The need for rapidand accurate sequencing of human genome has resulted in the introduction ofnext generation sequencing （NGS） technology. NGS refers to the second-generation DNA sequencing technologies where millions of DNA can besequenced simultaneously. Some of the next gen sequencing methods employedare Roche/454 life science, Illumina/Solexa, SOLiD system and HeliScope.Application of NGS in decoding the genomic database of various oral diseasesmay possess therapeutic and prognostic value. This presentation provides anoverview of the basics of NGS and their potential applications in oral diseasediagnostics.

关 键 词：Molecular diagnostics Next generation sequencing ILLUMINA Oral diseases Oral cancer 

分 类 号：R282.71[医药卫生—中药学] R394[医药卫生—中医学]