蚣甲油剂对深Ⅱ度烫伤白兔创面愈合及其肿瘤坏死因子α和白介素8表达的影响  

Impact of the scolopendra-manis pentadactyla linnaeus oil on wound healing and expression of tumor necrosis factor-α and interleukin-8 in rabbits with deep Ⅱ scald wound

作　　者：杨媚月[1] 张文兴[1] 刘秀芝[2] 夏传林[2] YANG Mei-yue;ZHANG Wen-xing;LIU Xiu-zhi(The First Affiliated Hospital of Hunan Traditional Chinese Medicine University,Changsha 410007,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南省长沙市410007 [2]湖南中医药大学

出　　处：《临床合理用药杂志》2018年第30期30-32,共3页Chinese Journal of Clinical Rational Drug Use

基　　金：湖南省中医药管理局基金项目(201781)

摘　　要：目的观察蚣甲油剂对深Ⅱ度烫伤白兔创面愈合及其肿瘤坏死因子α(TNF-α)、白介素8(IL-8)表达的影响。方法由湖南中医药大学实验室提供健康中国白兔30只,于其背部脊柱两侧建立2个约3 cm圆形深Ⅱ度烫伤模型并经组织学检查证实。随机分为对照组和观察组,各15只。对照组创面采用湿润烧伤膏纱布覆盖包扎,观察组创面采用蚣甲油剂纱布覆盖包扎。分别于创面局部处理后第3、7、14、21天,比较两组白兔创面愈合率,并分别于创面局部处理后第1、3、7、14、21天比较切片组织TNF-α、IL-8表达情况。结果创面局部处理后第3、21天两组白兔创面愈合率比较,差异无统计学意义(P>0.05);术后第7、14天观察组创面愈合率高于对照组(P<0.05)。创面局部处理后第1、21天两组白兔TNF-α、IL-8比较,第14天IL-8比较,差异无统计学意义(P>0.05);创面局部处理后第3、7、14天观察组TNF-α低于对照组,第3、7天观察组IL-8低于对照组(P<0.05)。结论蚣甲油剂能有效抑制深Ⅱ度烫伤白兔创面早中期TNF-α和IL-8的表达,减弱局部炎性反应,避免烧伤后创面进一步加深,从而促进创面愈合。Objective To observe the impact of the scolopendra-manis pentadactyla linnaeus oil on wound healing and expression of tumor necrosis factor-α（TNF-α） and interleukin-8（IL-8） in rabbits with deep Ⅱ scald wound.Methods A total of 30 healthy Chinese white rabbits were provided by the Laboratory of Hunan Traditional Chinese Medicine University,two 3 cm deep round second degree scald models were established on both sides of the back spine and confirmed by histological examination,which were randomly divided into control group and observation group,15 rabbits in each group.The wounds in the control group were covered with MEBO and the wounds in the observation group were covered with oyster nail oil gauze.The wound healing rates of two groups were compared between 3 rd,7 th,14 th and 21 st after local treatment.The expression of TNF-α and IL-8 were compared on the 1 st,3 rd,7 th,14 th and 21 st days after local wound treatment.ResultsThere was no significant difference in the wound healing rate between the two groups on the 3 rd and 21 st day after local treatment（P0.05）,and the wound healing rate in the observation group was higher than control group on the 7 th and 14 th day after operation（P0.05）.There was no significant difference in the levels of TNF-α and IL-8 between the two groups on the 14 th day（P0.05）,but on the 3 rd,7 th and 14 th day after local wound treatment,the level of TNF-α in observation group was lower than control group,and on the 3 rd and 7 th day the level of IL-8 in observation group was lower than control group（P0.05）.Conclusion The scolopendra-manis pentadactyla linnaeus oil preparation can effectively inhibit the burn wounds TNF-α,the expression of IL-8,so as to reduce inflammation,and promote wound healing.

关 键 词：创伤和损伤 蚣甲油剂 深Ⅱ度烫伤创面 肿瘤坏死因子Α 白介素8 

分 类 号：R285.5[医药卫生—中药学]