紫檀茋抗急性肾缺血再灌注损伤的作用及机制  

Protective effects of sandalwood on acute renal ischemia-reperfusion injury and its mechanism

作　　者：张珊珊[1] 高丹[1] 吴歌[1] Zhang Shanshan;Gao Dan;Wu Ge(Department of Nephrology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院肾内科,450052

出　　处：《中国实用医刊》2018年第20期1-4,7,共5页Chinese Journal of Practical Medicine

基　　金：河南省高等学校重点科研项目（15A320033）

摘　　要：目的 探讨紫檀芪（Pter）是否具有抗急性肾缺血再灌注损伤（IRI）作用及其可能发生机制.方法 选取雄性SD大鼠30只,随机分为假手术组（Sham组,n=10）、肾缺血再灌注组（IRI组,n=10）和紫檀芪治疗组（IRI＋Pter治疗组,n=10）.通过夹闭双肾动脉建立大鼠肾脏急性缺血再灌注模型,2周后检测大鼠血清肌酐、尿素氮水平,超氧化物歧化酶（SOD）活性、肿瘤坏死因子-α（TNF-α）水平以及caspase-3活性和核因子（NF-κB）的表达水平.结果 Pter能有效改善急性肾缺血再灌注损伤后肾功能,与未治疗的IRI组比较,急性肾IRI大鼠接受Pter治疗后,体内SOD活性升高（P〈0.01）,TNF-α表达水平降低（P〈0.01）.另外,Pter可抑制急性肾IRI诱导的caspase-3活化和NF-κB的表达（P〈0.01）.结论 Pter可能通过抗氧化应激、减轻炎症反应、抗细胞凋亡和抑制TLR4/NF-κB信号传导通路起到防止急性肾IRI的作用.Objective To investigate whether Pter protects against acute renal ischemia reperfusion in rats and its possible mechanism .Methods Thirty adult male Sprague Dawley rats were randomly divided into the following 3 groups：Sham group （ n=10 ） , IRI group （ n=10 ） and IRI＋Pter group （ n=10 ） .The bilateral arteries were bloked to make the IRI models .Serum nitrogen and creatinine were evaluated after 2 weeks.The concentrations of SOD and TNF-αwere measured by ELISA .The caspase 3 activity and the nuclear factor （ NF-κB ） expression were detected by immunohistochemistry . Results Pter treatment improved renal function following acute renal IRI .Compared with IRI group , SOD expression levels were significantly increased （ P 〈0.01 ） and TNF-αexpression levels were significantly decreased （ P〈0.01） following treatment with Pter in acute renal IRI rats .In addition, Pter significantly attenuated caspase-3 activity and the nuclear factor （ NF-κB ） expression （ P 〈0.01 ） . Conclusions Pter may prevent acute renal IRI through antioxidant stress , alleviating inflammation , antiapoptosis and inhibiting TLR 4/NF-κB signaling pathway .

关 键 词：急性缺血再灌注损伤 紫檀茋 氧化应激 炎症反应 

分 类 号：R285.5[医药卫生—中药学]