成纤维细胞因子对急性胰腺炎家兔胃肠动力障碍的影响  

Effect of fibroblast factor on gastrointestinal motility disorder in rabbits with acute pancreatitis

作　　者：韩俊岭[1] 王刚[1] 王放[1] 王增允 孙腾飞 连颖 王丽君[1] HAN Jun-ling;WANG Gang;WANG Fang;WANG Zeng-yun;SUN Teng-fei;LIAN Ying;WANG Li-jun(Department of Gastroenterology,Qinhuangdao First Hospital,Qinhuangdao,Hebei 066000,China)

机构地区：[1]秦皇岛市第一医院消化内科,河北秦皇岛066000

出　　处：《中国临床研究》2018年第11期1470-1474,共5页Chinese Journal of Clinical Research

基　　金：2017年秦皇岛市市级科技计划重点研发项目(201703A055)

摘　　要：目的研究成纤维细胞因子c-kit水平对急性胰腺炎(AP)家兔胃肠动力障碍的影响。方法选择30只白兔随机分为假手术组和AP组,每组各15只。AP组白兔通过胰腺直接注入5%牛磺胆酸钠建立AP模型,假手术组白兔手术中仅轻轻翻动十二指肠。采用放射免疫法检测手术前后血浆胃泌素(GAS)、胃动素(MTL)、胆囊收缩素(CCK)含量,采用Oddi括约肌测压法检测手术前后Oddi括约肌功能。取胰腺组织制作病理切片,光学显微镜下观察胰腺病理变化,逆转录聚合酶链反应(RT-PCR)法检测Oddi括约肌中c-kit mRNA相对表达量,Western blot检测c-kit蛋白表达量。结果光镜下显示AP组胰腺组织叶间隙增宽,细胞水肿,部分腺泡细胞坏死,并有大量炎性细胞浸润;假手术组无明显病理改变。术后,假手术组Oddi括约肌功能指标较术前无统计学差异(P> 0. 05),AP组Oddi括约肌基础压和收缩频率较术前均降低,且低于假手术组(P <0. 01)。术后,假手术组胃肠激素各指标较术前差异无统计学意义(P> 0. 05),AP组血浆GAS高于术前,MTL、CCK低于术前,且GAS高于假手术组,MTL、CCK低于假手术组,差异有统计学意义(P <0. 01)。AP组Oddi括约肌中c-kit mRNA相对表达量(0. 76±0. 22)低于假手术组(1. 39±0. 36),差异有统计学意义(t=5. 783,P <0. 01);AP组Oddi括约肌中c-kit蛋白表达(0. 60±0. 18)低于假手术组(1. 13±0. 33),差异有统计学意义(t=5. 461,P <0. 01)。结论 AP白兔Oddi括约肌中c-kit表达减弱,胃肠激素失调,从而引起胃肠动力障碍。objective To study the effect of level of c-kit,a fibroblast factor,on gastrointestinal motility disorder in rabbits with acute pancreatitis（AP）. Methods Thirty white rabbits were randomly divided into sham operation group and AP group（n = 15 each）. In AP group,AP model was established by direct injection of 5% sodium taurocholate into the pancreas. In sham operation group,the duodenum was lightly turned only during the operation. Radioimmunoassay was used to detect the contents of plasma gastrin（GAS）,motilin（MTL） and cholecystokinin（CCK） before and after operation. Oddi sphincter manometry method was used to detect Oddi sphincter functions before and after operation. Pancreatic tissue was taken to make pathological sections,and pathological changes of pancreas were observed under optical microscope. Reverse transcription-polymerase chain reaction（RT-PCR） was used to detect the relative expression quantity of c-kit mRNA in Oddi sphincter,and Western blot method was used to detect the relative expression quantity of c-kit protein in Oddi sphincter. Results Pathological examination showed widening of the interlobar space of pancreatic tissues,cell edema,partial acinar cells necrosis and a large number of inflammatory cells infiltration under microscopy in AP group,while there was no obvious pathological changes in sham operation group. In sham operation group. there were no significant differences in indexes of Oddi sphincter functions before operation and after operation（all P〉0. 05）. In AP group,the basal pressure and contraction frequency of Oddi sphincter after operation were significantly lower than those in pre-operation and were significantly lower than those in sham operation group（all P〈0. 01）. In sham operation group,there were no significant differences in indexes of gastrointestinal hormones before operation and after operation（all P〉0. 05）. In AP group,the level of plasma GAS after operation was significantly higher than that in pre-operation and higher than th

关 键 词：急性胰腺炎 胃肠动力障碍 成纤维细胞因子 C-KIT 胃肠激素 ODDI括约肌 

分 类 号：R657.51[医药卫生—外科学]