G蛋白偶联雌激素受体通过改善肾叶间动脉舒缩活动减轻肾脏缺血再灌注损伤  

G protein- coupled estrogen receptor reduces renal ischemia- reperfusion injury by improving diastolic function of renal interlobular artery

作　　者：常越辰 韩子伟 周颖 张洋 冯子奕 马克涛[1] 张志琴[2] 李丽[1] 司军强[1] Chang Yuechen;Hart Ziwei;Zhou Ying;Zhang Yang;Feng Ziyi;Ma Ketao;Zhang Zhiqin;Li Li;Si Junqiang(Department of Physiolog;Shihezi University Medical College,Xinjiang Shihezi 832000,China)

机构地区：[1]新疆石河子大学医学院基础医学系,832000 [2]浙江金华职业技术学院医学院基础医学机能课程组,321017

出　　处：《中华肾脏病杂志》2018年第11期838-844,共7页Chinese Journal of Nephrology

基　　金：国家自然科学基金（81560175、81260159）

摘　　要：目的探讨G蛋白偶联雌激素受体（G protein—coupled estrogen receptor，GPER）对肾叶间动脉舒缩功能的影响，及其减轻大鼠肾脏缺血再灌注损伤的作用。方法雌性去卵巢大鼠被分为对照组；缺血再灌注损伤（IRI）组；GPER特异性激动剂（G1）干预组；GPER特异性阻断剂＋GPER特异性激动剂（G15＋G1）干预组。采用组织病理学检查（HE染色）、肾功能检测及Paller评分等方法鉴定模型成功与否及肾脏损伤程度；离体微血管压力直径测定仪检测各组大鼠肾叶间动脉舒缩活动情况；免疫荧光技术观察GPER在肾叶间动脉上的表达；Western印迹法检测各组大鼠肾叶间动脉GPER蛋白表达量；硝酸还原酶法检测血清NO含量。结果与IRI组比较，G1干预组大鼠血BUN、Scr水平及Paller评分明显降低（均P〈0．05）；肾叶间动脉收缩率明显升高[（40．76±1．57）％比（29．78±1.87）％，P〈0.05]。免疫荧光结果冠示，GPER在肾叶间动脉平滑肌细胞及内皮细胞中表达，且IRI组较对照组表达增多；G15＋G1干预组较G1干预组表达降低（均P〈0．05）。与IRI组比较，G1干预组大鼠血清NO含量显著上升（均P〈0．05）。结论肾脏缺血再灌注损伤过程中GPER可能通过提高NO含量，从而调节肾叶问动脉的舒缩活动，减轻肾缺血再灌注损伤。Objective To investigate the effect of G protein- coupled estrogen receptor （GPER） on the diastolic function of renal interlobular artery and reduce renal ischemia-repcffusion injury in rats. Methods Female ovarieetomized rats were divided into control group; ischemia- repeffusion injury （IRI） group; GPER-speeific agonist （G1） intervention group; GPER-specific blocker＋ GPER-specific agonist （G15＋G1） intervention group. Histopalhological examination （HE staining）, renal function test and Paller score were used to identify the success of the model and the degree of kidney damage. In vitro mierovascular pressure diameter measuring instrument was used to detect the relaxation and contraction activity of renal interlobular artery in each group. Immunofluorescence technique was used to observe the expression of GPER on the renal interlobular artery. Western blotting was used to detect the expression of GPER protein in renal interlobular artery of rats in each group. The NO content was determined by a nitrate reductase method. Results Compared with IRI group, serum BUN, Scr level and Paller score in G1 intervention group were significantly decreased （all P 〈 0.05）. The systolic rate of renal interlobar artery was significantly increased [（40.76±1.57）% vs （29.78±1.87）%, P 〈 0.05]. The results of immunofluorescence showed that GPER was expressed in renal interlobular artery smooth muscle cells and endothelial cells , and the expression of IRI group was higher than that of the control group. The expression of G15＋G1 intervention group was lower than that of G1 intervention group （all P 〈 0.05）. Compared with the IRI group, the NO content in the G1 intervention group increased significantly （all P 〈 0.05）. Conclusions During renal ischemia- reperfusion injury, GPER may regulate the systolic and diastolic activity of the renal interlobar artery by increasing the content of NO, so as to alleviate the renal ischemia-reperfusion injury.

关 键 词：再灌注损伤 受体 雌激素 一氧化氮 肾叶间动脉 

分 类 号：R692[医药卫生—泌尿科学]