凋膜止崩液对体外培养人子宫内膜腺上皮细胞Bcl-2/Bax蛋白及基因表达的影响  

Effects of the Diaomo-Zhibeng liquid on the expression of Bcl-2/Bax mRNA and their protein in vitro human endometrial glandular epithelial cells

作　　者：李楠 徐翠[2] 贺丰杰 谈勇 王灿灿 Li Nan;Xu Cui;He Fengjie;Tan Yong;Wang Cancan(Postdoctoral of Grade 2014,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210046,China)

机构地区：[1]南京中医药大学附属医院生殖医学科,210046 [2]北京中医药大学东方医院妇科,100078 [3]陕西中医药大学附属医院妇科,咸阳712000

出　　处：《国际中医中药杂志》2018年第11期1065-1069,共5页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金（81173290）;陕西省“13115”科技创新工程重大科技专项（2013KTCQ3-11）

摘　　要：目的 观察凋膜止崩液对体外培养人子宫内膜增生症患者内膜腺上皮细胞Bcl-2/Bax蛋白及mRNA表达的影响。方法 收集病理诊断为子宫内膜增生症患者的子宫内膜组织，经分离、培养、鉴定后，按照随机数字表法将腺上皮细胞分为分为空白对照组，凋膜止崩液低、中、高剂量组。空白对照组加入含10%FBS培养液，凋膜止崩液低、中、高剂量组在含10%FBS的细胞培养液中分别加入12.5、25、50 μg/ml的凋膜止崩液。干预后48 h，采用蛋白免疫印迹法及RT-PCR法检测腺上皮细胞Bcl-2、Bax蛋白及mRNA表达。结果 与空白对照组比较，凋膜止崩液低、中、高剂量组Bcl-2 mRNA[（1.51±0.07）、（1.09±0.06）、（0.93±0.07）比（1.92±0.08）]及蛋白[（0.91±0.02）、（0.72±0.03）、（0.62±0.03）比（1.28±0.02）]表达降低（P＜0.05），Bax mRNA[（5.73±0.37）、（8.00±0.23）、（10.72±0.40）比（3.27±0.34）]及蛋白[（0.45±0.02）、（0.63±0.02）、（0.78±0.03）比（0.32±0.02）]表达升高（P＜0.05）。与低剂量组比较，中、高剂量组Bcl-2 mRNA及蛋白表达明显降低（P＜0.05），Bax mRNA及蛋白表达明显升高（P＜0.05）。结论 凋膜止崩液可增加腺上皮细胞对凋亡的易感性，促进细胞凋亡。Objective To observe the effects of the Diaomo-Zhibeng liquid on the expression of Bcl-2/Bax mRNA and their protein in vitro endometrial glandular epithelial cells in patients of endometrial hyperplasia Methods The endometrial tissue in patients with the pathological diagnosis of endometrial hyperplasia were collected. After isolation, culture and identification, the endometrial cells were divided into four groups： low dosage group（12.5 μg/ml）, middle dosage group（25 μg/ml）, high dosage group（50 μg/ml） and blank control group at randomaccording to the random number table. Three duplicate samples were set for each group. After 48h, the expression of Bcl-2 and Bax were detected by RT-PCR and Western-blot. Results Compared with the blank control group, the expression of Bcl-2 mRNA （1.51 ± 0.07, 1.09 ± 0.06, 0.93 ± 0.07 vs. 1.92 ± 0.08） and protein （0.91 ± 0.02, 0.72 ± 0.03, 0.62 ± 0.03 vs. 1.28 ± 0.02） significantly decreased, while the expression of Bax mRNA （5.73 ± 0.37, 8.00 ± 0.23, 10.72 ± 0.40 vs. 3.27 ± 0.34） and protein （0.45 ± 0.02, 0.63 ± 0.02, 0.78 ± 0.03 vs. 0.32 ± 0.02） sifnificantly promoted in low dosage group, medium dosage group and high dosage group of Diaomo-Zhibeng liquid （P〈0.05）. Compared with low dosage group, the expression of Bcl-2 mRNA and protein significantly decreased in middle and high dose group （P〈0.05）, but Bax mRNA and protein significantly increased （P〈0.05）. Conclusions The Diaomo-Zhibeng liquid can increase the susceptibility of epithelial cells to apoptosis, regulate the balance of cell proliferation and apoptosis tends to apoptosis, have the effect of accelerating cells apoptosis.

关 键 词：子宫内膜增生 细胞凋亡 基因 BCL-2 bcl-2-相关x蛋白 凋膜止崩液 

分 类 号：R285[医药卫生—中药学]