绵羊EPAS1基因Exon12和Exon16 SNP多态位点筛选  

Screening of SNP Polymorphic Loci of Exon12 and Exon16 of EPAS1 Gene in Sheep

作　　者：穷达[1] 卓嘎 巴桑 唐晓惠[1] 强巴央宗[1] 张浩[2] Qiongda;Zhuga;Basang;TANG Xiaohui;Qiangba Yangzong;ZHANG Hao(Key Laboratory of Animal Breeding and Reproduction of Tibet Autonomous Region,Tibet Agriculture and Animal Husbandry University,Nyingchi Tibet,860000,China;College of Animal Science and Technology,China Agriculture University,Beijing 100094,China)

机构地区：[1]西藏农牧学院西藏自治区动物遗传育种与繁殖重点实验室,西藏林芝860000 [2]中国农业大学动物科学技术学院,北京100193

出　　处：《高原农业》2018年第5期503-506,共4页Journal of Plateau Agriculture

基　　金：西藏自治区科技厅自然基金(厅校联合基金)项目(2016ZR-NY-01);西藏自治区教育厅高校青年教师创新支持计划QCZ2016-52

摘　　要：为了筛选绵羊EPAS1基因SNP多态位点,本文设计2对引物利用PCR技术从5个绵羊品种(3个高原品种和2个低地品种)基因组中扩增了EPAS1基因exon12和exon16片段,测序结果发现高原绵羊和低地绵羊群体间存在4个SNPs位点,其中1个位于第12外显子(276 T>C),3个位于第16外显子(904 T>C、914A>C和977IndelA),这些多态性位点可能对EPAS1基因的功能具有重要的调控作用,这为藏绵羊高原低氧适应机制研究提供了基础。In order to screen SNP polymorphic loci of the sheep EPAS1 gene, two pairs of primers were designed to amplify Exon12 and Exon16 fragments of EPAS1 gene from genomes of five sheep varieties（three highland varieties and two lowland varieties） using PCR technology. The sequencing results showed there were four SNPs between the Tibetan and lowland sheep, in which, one SNP（276 TC） was in exon 12, and three SNPs（904 TC, 914 AC and 977 IndelA） in exon 16. These polymorphisms may have important regulatory effects on the function of EPAS1 gene, which provided a basis for study of the adaptation mechanism of hypoxia in sheep.

关 键 词：EPAS1 SNP多态位点 低氧适应性 藏绵羊 

分 类 号：S826[农业科学—畜牧学]