小麦植株中叶菌唑残留测定方法  

Determination of Residual Metconazole in Wheat Plant

作　　者：李如美[1] 戴争[1] 李瑞娟[1] 李丹丹 高宗军[1] Li Ru-mei;Dai Zheng;Li Rui-juan;Li Dan-dan;Gao Zong-jun＊(Key Laboratory for Plant Virology of Shandong,Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]山东省农业科学院植物保护研究所山东省植物病毒学重点实验室,济南250100

出　　处：《现代农药》2018年第6期45-47,共3页MODERN AGROCHEMICALS

基　　金：国家重点研发计划"化学农药协同增效技术及产品研发"(2016YFD0200500);山东省农业科学院创新工程项目

摘　　要：采用高效液相色谱建立小麦植株中叶菌唑残留测定方法。分别以乙腈、二氯甲烷为提取溶剂,通过Bond Elut Carbon固相萃取柱净化,SB-C_(18)色谱柱分离,Agilent 1260高效液相色谱仪测定。结果表明,当叶菌唑质量浓度为0.1～10.0 mg/L时,其质量浓度与色谱峰面积呈线性关系,相关系数为0.999 9。乙腈超声离心法的平均回收率为90.40%～98.39%,相对标准偏差为1.62%～5.10%;二氯甲烷萃取法的平均回收率为91.34%～95.00%,相对标准偏差为0.86%～3.89%。2种试验方法均容易操作,且灵敏度高。To analyze the feasibility of metconazole residues in wheat with acetonitrile ultrasonic centrifugation extraction method and dichloromethane extraction method, the samples were extracted by acetonitrile or dichloromethane, purified by the Bond Elut Carbon extraction column, separated by SB-C18 chromatographic column, then determinated by HPLC. The results showed that there was a significant linear relationship between the concentration of metconazole and the chromatographic peak area at the concentration of 0.1-10.0 mg/L. The average recoveries of acetonitrile ultrasonic centrifugation extraction method were 90.40%-98.39%, and the relative standard deviations were 1.62%-5.10%. The average recoveries of dichloromethane extraction method were 91.34%-95.00%, and the relative standard deviations were 0.86%-3.89%. Two methods were easy to operate and had high sensitivity.

关 键 词：叶菌唑 残留 小麦植株 提取方法 高效液相色谱 测定 

分 类 号：TQ450.263[化学工程—农药化工]