三七总皂苷对脂多糖诱导的THP-1源巨噬细胞炎症损伤的保护作用  

Protective effects of panax notoginseng saponins against the lipopolysaccharide-induced inflammatory injury in THP-1 macrophages

作　　者：周莹[1] 李建军[1] 黄巧娟[1] ZHOU Ying;LI Jian-jun;HUANG Qiao-juan(The Second Affiliated Hospital of Catangxi Medical University,Narming 530007,China)

机构地区：[1]广西医科大学第二附属医院,南宁530007

出　　处：《中国临床药理学杂志》2018年第22期2631-2635,共5页The Chinese Journal of Clinical Pharmacology

基　　金：广西中医药民族医药自筹经费科研课题基金资助项目(GZZC14-50)

摘　　要：目的探讨三七总皂苷(PNS)对脂多糖(LPS)诱导的THP-1源巨噬细胞炎症损伤的保护作用。方法用佛波酯(PMA)诱导THP-1源巨噬细胞,制作成炎症细胞模型。实验分为空白组、对照组和实验A、B、C、D、E组。空白组予以正常培养液培养24 h,对照组予以含1μg·m L^(-1)LPS的培养液培养24 h,实验A、B、C、D、E组在1μg·m L^(-1)LPS刺激24 h的基础上,分别加入12. 5,25. 0,50. 0,100. 0,200. 0μg·m L^(-1)PNS培养24 h。用CCK-8法检测细胞增殖,用酶联免疫吸附法检测细胞上清液白细胞介素-1β(IL-1β)、IL-6和肿瘤坏死因子-α(TNF-α)的分泌水平,用实时荧光聚合酶链式反应法检测IL-1β、IL-6和TNF-α的mRNA表达水平。结果实验组细胞结构形态与空白组相似,细胞器状态均在正常范围内。实验A、B、C、D、E组及空白组和对照组的IL-1β分泌水平分别为(251. 00±8. 00),(200. 00±4. 00),(208. 00±4. 00),(260. 00±4. 00),(272. 00±2. 00),(203. 00±6. 00)和(294. 00±4. 00)pg·L^(-1),IL-6分泌水平分别为(21. 30±1. 50),(23. 50±1. 00),(19. 20±1. 00),(23. 00±2. 00),(24. 10±0. 50),(14. 50±1. 00)和(26. 50±1. 50) pg·L^(-1),TNF-α分泌水平分别为(2214. 00±10. 00),(1985. 00±10. 00),(1346. 00±20. 00),(2941. 00±40. 00),(3851. 00±40. 00),(1251. 00±10. 00)和(5013. 00±10. 00) pg·L^(-1),IL-1βmRNA表达水平分别为(0. 12±0. 01),(0. 02±0. 01),(0. 02±0. 01),(0. 04±0. 01),(0. 22±0. 02),(0. 02±0. 01)和(0. 96±0. 02),IL-6 mRNA表达水平分别为(0. 45±0. 01),(0. 34±0. 01),(0. 05±0. 01),(0. 30±0. 01),(0. 78±0. 01),(0. 03±0. 01)和(0. 98±0. 01),TNF-αmRNA表达水平分别为(0. 44±0. 01),(0. 22±0. 01),(0. 03±0. 01),(0. 03±0. 01),(0. 24±0. 01),(0. 02±0. 01)和(0. 95±0. 01),C组的上述指标与对照组比较,差异均有统计学意义(均P <0. 05)。结论 PNS对LPS诱导THP-1源巨噬细胞的炎症损伤有保护作用。Objective To investigate the protective effect of panax noto- ginseng saponins （PNS） on inflammatory damage of THP - 1 macropha- ges induced by lipopolysaccharide （LPS）. Methods THP - 1 macro- phages was induced by phorbol ester （PMA） , and established inflamma- tory cell model. The experiment was divided into blank group, control group and test A, B, C, D, E groups. Blank group was were cultured in normal culture medium for 24 h. Control group was cultured in medium containing 1 μg·mL^-1 LPS for 24 h. On the basis of stimulation of the 1 μg·mL^-1 LPS for 24 h, test A, B, C, D, E groups were given 12.5, 25.0, 50. 0, 100. 0,200. 0 μg·mL^-1 PNS, respectively. Cell proliferation was detected by CCK - 8 assay. The protein secretion levels of interleukin - 1β（ IL - 1β）, IL - 6 and tumor necrosis factor -α（ TNF -α） in supernatant were measured by enzyme - linked immunosorbent assay （ELISA）. The mRNA expression levels of IL - 1β, IL - 6 and TNF -α were detected by real - time fluores- cence polymerase chain reaction. Results The structure and morphology of the cells in the test group were similar to those in the blank group, and the status of organelles in the test group was within the normal range. The main indexes of test A, B, C, D, E groups, blank group and eontrol group were compared ： the levels of IL - 1β secretion were. （251.00 ± 8.00）, （ 200. 00 ± 4.00 ）, （ 208.00 ± 4. 00）, （ 260. 00 ± 4.00 ）, （ 272.00 ± 2.00 ）, （ 203.00 ± 6.00 ） and （294.00±4.00） pg ·L^-1, the levels of IL - 6 secretion were （21.30±1.50）, （23.50± 1.00）, （19.20±1.00）, （23.00±2.00）,（24.10±0.50）, （14.50±1.00） and （26.50±1.50）pg ·L^-1, the levels of TNF -α secretion were （ 2214. 00 ± 10. 00 ）, （ 1985.00 ± 10. 00 ）, （ 1346.00 ±20. 00 ）, （ 2941.00 ± 40. 00 ）, （3851.00 ± 40. 00 ）, （ 1251.00 ±10. 00 ） and （ 5013.00 ± 10.00 ） pg ·L^-1, the expression levels of IL - 1β mRNA were （0.12±0.01）, （0.02±0

关 键 词：三七总皂苷 脂多糖 炎症损伤 

分 类 号：R28[医药卫生—中药学]