针灸上调血清Aβ内化酶对阿尔茨海默病大鼠学习记忆能力和β淀粉样蛋白沉积的影响  

Acupuncture and Moxibustion Improves Learning-memory Ability of Alzheimer's Disease Rats Possibly by Up-regulating Serum Aβ Internalization Enzyme Contents

作　　者：汤双红 杜艳军 肖佳欢 王芸 沈峰 孙国杰 TANG Shuang-hong;DU Yan-jun;XIAO Jia huan;WANG Yun;SHEN Feng;SUN Guo-jie(College of Acupuncture-mozibustion and Orthopedics,Hubei University of Chinese Medicine,Wuhan 430061,China;Hubei Provincial Collaborative Innovation Center for Preventive Treatment of Diseases with Acupuncture and Moxibustion,Wuhan 430061)

机构地区：[1]湖北中医药大学针灸骨伤学院,武汉430061 [2]针灸治未病湖北省协同创新中心,武汉430061

出　　处：《针刺研究》2018年第11期692-697,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81473786;81173323)

摘　　要：目的:探讨针灸通过降解β淀粉样蛋白(Aβ)改善阿尔茨海默病(AD)大鼠学习记忆能力的作用机制。方法:Wistar大鼠随机分为正常组、假手术组、模型组和针灸组,每组10只。采用双侧海马注射5μL Aβ_(1-42)复制AD模型。针灸组"百会"及"肾俞"穴针刺加艾灸治疗,15min/次,1次/d,6d为1个疗程,治疗2个疗程。采用Morris水迷宫检测各组大鼠行为学,ELISA法检测血清Aβ_(1-42)及Aβ内化酶的含量,免疫组化法检测海马齿状回内Aβ_(1-42)表达变化。结果:与假手术组比较,模型组前5d及后3d平均逃避潜伏期明显延长,有效区停留时间明显缩短,跨越原平台位置的次数明显减少(P<0.01),血清中转甲状腺素蛋白(TTR)、脂蛋白酯酶(LPL)、α_2巨球蛋白(α_2M)及载脂蛋白E(ApoE)含量明显下降(P<0.01,P<0.05),Aβ_(1-42)的含量明显增多(P<0.01),齿状回内Aβ_(1-42)阳性表达升高(P<0.01)。与模型组比较,针灸组前5d及后3d平均逃避潜伏期明显缩短,有效区停留时间明显延长,跨越原平台位置的次数明显增多(P<0.01,P<0.05),血清中TTR、LPL、α_2M及ApoE含量明显上升(P<0.01,P<0.05),Aβ_(1-42)含量明显减少(P<0.05),齿状回内Aβ_(1-42)阳性表达降低(P<0.05)。结论:针灸"百会""肾俞"能明显改善AD大鼠学习记忆能力,其机制可能与上调血清中Aβ内化酶的含量,促进Aβ的清除以保护神经细胞有关。Objective To observe the effect of acupuncture and moxibustion（AM）on learning-memory ability and expression of amyloid beta（Aβ）in the hippocampal dentate gyrus（DG）of Alzheimers disease（AD）rats,so as to explore its mechanism underlying improvement of AD. Method Forty male Wistar rats were randomly divided into normal,sham operation,model and AM groups（n=10 in each）.The AD model was established by bilateral hippocampal injection of Aβ_（1-42）（5 μL）.The AM was applied at＂Baihui＂（GV 20）and＂Shenshu＂（BL 23）for 15 min,once daily for 12 times.Morris water maze tests were used to assess the rats＇ learning-memory ability.The levels of serum Aβ_（1-42） and Aβinternalizing enzymes including transthyretin（TTR）,lipoprotein lipase（LPL）,alpha 2 macroglobulin（α_2 M）and apolipoprotein E（ApoE）were detected by ELISA.The expression of Aβ_（1-42） in the hippocampal DG was detected by immunohistochemistry.Results Compared with the sham operation group,the average escape latency of location navigation test was significantly prolonged in the first 5 days and the last 3 days（P〈0.01）,and the residence time of the target-quadrant and the platform crossing times of special probing task were significantly reduced in the model group（P〈0.01）.After the intervention,the average escape latency was obviously shortened in the first 5 days and the last 3 days（P〈0.01）,and both the residence time and the platform crossing times were significantly increased in the AM group relevant to the model group（P〈0.01,P〈0.05）.After modeling,the contents of TTR,LPL,α_2 M and ApoE in the serum were significantly lower in the model group than in the sham operation group（P〈0.01,P〈0.05）,while the levels of serum Aβ_（1-42） and hippocampal Aβ_（1-42） expression were considerably increased relevant to the sham operation group（P〈0.01）.Following the intervention,the TTR,LPL,α_2 M and ApoE contents in the serum were significantly increased（P〈0.0

关 键 词：阿尔茨海默病 针灸疗法 学习记忆能力 海马齿状回 β-淀粉样蛋白 血清Aβ内化酶 

分 类 号：R245.8[医药卫生—针灸推拿学]