尿酸对大鼠血管平滑肌细胞赖氨酰氧化酶和基质金属蛋白酶-2表达的影响  

Effects of Uric Acid on Expression of Lysyl Oxidase and Matrix Metalloproteinase-2 in Rat Smooth Muscle Cells

作　　者：刘会[1] 张曙影[2] 袁国军 荣季冬[4] LIU Hui;ZHANG Shu-ying;YUAN Guo-jun;RONG Ji-dong(Department of Cardiology,Guizhou Provincial People＇s Hospital,Guiyang（550002）,Guizhou,China)

机构地区：[1]贵州省人民医院心内科,贵阳市550002 [2]大连大学附属中山医院心内科 [3]萍乡市人民医院心内科 [4]遵义医学院附属医院心内科

出　　处：《中国循环杂志》2018年第11期1113-1117,共5页Chinese Circulation Journal

基　　金：贵州省科学技术厅临床研究中心项目(黔科合平台人才(2017)5405)

摘　　要：目的:研究尿酸对大鼠血管平滑肌细胞赖安酰氧化酶(LOX)和基质金属蛋白酶-2(MMP-2)表达的影响。方法:人工传代培养大鼠血管平滑肌细胞,分为对照组、尿酸组(20、40、60 mg/L尿酸干预48 h;40 mg/L尿酸干预24、48、72 h)、β氨基丙腈组(40 mg/L尿酸干预48 h后加入LOX的特异性抑制剂β氨基丙腈10 mg/ml孵育24 h)。用激光共聚焦显微镜观察细胞内活性氧情况。提取大鼠血管平滑肌细胞RNA和蛋白,用逆转录聚合酶链式反应和免疫印迹法测定LOX及MMP-2信使核糖核酸(mRNA)和蛋白的表达水平。结果:尿酸组大鼠血管平滑肌细胞增殖量、细胞内活性氧、LOX及MMP-2的m RNA和蛋白水平均明显高于对照组;与尿酸组相比,β氨基丙腈组大鼠血管平滑肌细胞LOX及MMP-2 mRNA和蛋白表达均明显下调,差异均有统计学意义(P均<0.01)。结论:尿酸可上调大鼠血管平滑肌细胞LOX及MMP-2表达。Objectives： To explore the effects of uric acid on expression of lysyl oxidase （LOX） and matrix metalloproteinase-2 （ MMP-2 ） in rat vascular smooth muscle cells. Methods： Rat vascular smooth muscle cell was cultured, and divided into following groups： control group, uric acid group （cells were treated with 20, 40, 60 mg/L uric acid for 48 hours, cells were treated with 40 mg/L uric acid for 24, 48, 72 h） and 13-aminopropionitrile group （cells were treated with 10 mg/ml β-aminopropionitrile for 24 hours）. The reactive oxygen species was detected by confocal microscopy, mRNAs and protein expression levels of LOX and MMP-2 in rat vascular smooth muscle cells were measured by RT-PCR and Westem blot, respectively. Results： The number of increased cell proliferation, reactive oxygen species burst, mRNAs and protein expression levels of LOX and MMP-2 were significantly increased in uric acid group than in control group （P〈0.01）. The mRNAs and protein expression levels of LOX and MMP-2 were significantly downregulated in β-aminopropionitrile group than in uric acid group （P〈0.01）. Conclusions： Uric acid can enhance the expression of LOX and MMP-2 in rat vascular smooth muscle cells.

关 键 词：尿酸 蛋氨酸-6-氧化酶 基质金属蛋白酶-2 血管平滑肌细胞 

分 类 号：R54[医药卫生—心血管疾病]